Catalog No:A7920
Host species:Rabbit
Purity:Affinity purification
ABclonal:Western blot - POU5F1 antibody (A7920)
ABclonal:Immunohistochemistry - POU5F1 antibody (A7920)
ABclonal:Immunofluorescence - POU5F1 antibody (A7920)

To buy,select size,quantity


This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Immunogen information

ImmunogenA synthetic peptide of human POU5F1
SequenceEmail for sequence
Gene ID5460
Swiss ProtQ01860
SynonymsOct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4
Calculated MW30kDa/38kDa
Observed MW50kDa


ReactivityHuman, Mouse, Rat
Tested applicationsWB, IHC, IF
Recommended DilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesNIH/3T3, Mouse brain, Mouse testis, Mouse ovary, Rat testis
Cellular LocationCytoplasm, Nucleus
Customer Validation

IHC (Human)

WB (Human)

POU5F1 Polyclonal Antibody images

ABclonal:Western blot - POU5F1 antibody (A7920)

Western blot - POU5F1 antibody (A7920)

Western blot analysis of extracts of various cell lines, using POU5F1 antibody (A7920) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - POU5F1 antibody (A7920)

Immunohistochemistry - POU5F1 antibody (A7920)

Immunohistochemistry of paraffin-embedded human brain cancer using POU5F1 antibody (A7920) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - POU5F1 antibody (A7920)

Immunofluorescence - POU5F1 antibody (A7920)

Immunofluorescence analysis of U2OS cells using POU5F1 antibody (A7920) at dilution of 1:100. Blue: DAPI for nuclear staining.

Submit an review for A7920

  1. Please choose your application ,enter the information requested below. All fields are mandatory, except the ones marked as optional.
  2. When you're happy with the review, submit it by clicking on the Submit button.
H(s)     M(s)
H(s)      M(s)     Second(s)
(allowed file types: .jpg, .png, .tif, .bmp, .gif)

Reviews List

Ask Us About This Product

submit your question on A7920 below

During business hours, we will respond to your email with in 24 hours. For any questions submitted on the weekend, a response will be received on Monday.

For immediate assistance during business hours M-F (excluding major holidays), please contact us.

Publishing research using A7920? Please let us know so that we can cite the reference in this datasheet.

You Viewed