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POP1 Polyclonal Antibody (A5961)

Datasheet

ABclonal:Western blot - POP1 Polyclonal Antibody (A5961)

Overview

Product namePOP1 Polyclonal Antibody
Catalog No.A5961
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the protein subunit of two different small nucleolar ribonucleoprotein complexes: the endoribonuclease for mitochondrial RNA processing complex and the ribonuclease P complex. The encoded protein is a ribonuclease that localizes to the nucleus and functions in pre-RNA processing. This protein is also an autoantigen in patients suffering from connective tissue diseases. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human POP1 (NP_055844.2).
SequenceMSNAKERKHAKKMRNQPTNVTLSSGFVADRGVKHHSGGEKPFQAQKQEPHPGTSRQRQTRVNPHSLPDPEVNEQSSSKGMFRKKGGWKAGPEGTSQEIPKYITASTFAQARAAEISAMLKAVTQKSSNSLVFQTLPRHMRRRAMSHNVKRLPRRLQEIAQKEAEKAVHQKKEHSKNKCHKARRCHMNRTLEFNRRQKKNIWLETHIWHAK
Gene ID10940
Swiss protQ99575
SynonymsPOP1; ANXD2; ribonucleases P/MRP protein subunit POP1
Calculated MW114kDa
Observed MW115kDa
ReactivityMouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMouse liver, Mouse kidney, Rat liver
Cellular locationNucleus, nucleolus
ABclonal:Western blot - POP1 Polyclonal Antibody (A5961) }

Western blot - POP1 Polyclonal Antibody (A5961)

Western blot analysis of extracts of various cell lines, using POP1 antibody (A5961) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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