Products > Catalog Antibodies > Polyclonal Antibodies

POLR2B Rabbit pAb (A5928)

Publications (3) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - POLR2B Polyclonal Antibody (A5928)

Western blot analysis of extracts of various cell lines, using POLR2B antibody (A5928) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - POLR2B Polyclonal Antibody (A5928)

Immunohistochemistry of paraffin-embedded rat liver using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - POLR2B Polyclonal Antibody (A5928)

Immunohistochemistry of paraffin-embedded rat brain using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - POLR2B Polyclonal Antibody (A5928)

Immunohistochemistry of paraffin-embedded mouse liver using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).

Overview

Product namePOLR2B Rabbit pAb
Catalog No.A5928
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2B (NP_000929.1).
SequenceMYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATL
Gene ID5431
Swiss protP30876
SynonymsPOLR2B; POL2RB; RPB2; hRPB140
Calculated MW133kDa
Observed MW134kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesSH-SY5Y, A375, HepG2, BT-474, Mouse brain, Mouse spleen, Mouse ovary, Rat ovary
Cellular locationNucleus
Customer validation

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - POLR2B Polyclonal Antibody (A5928) }

Western blot - POLR2B Polyclonal Antibody (A5928)

Western blot analysis of extracts of various cell lines, using POLR2B antibody (A5928) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - POLR2B Polyclonal Antibody (A5928) }

Immunohistochemistry - POLR2B Polyclonal Antibody (A5928)

Immunohistochemistry of paraffin-embedded rat liver using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - POLR2B Polyclonal Antibody (A5928) }

Immunohistochemistry - POLR2B Polyclonal Antibody (A5928)

Immunohistochemistry of paraffin-embedded rat brain using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - POLR2B Polyclonal Antibody (A5928) }

Immunohistochemistry - POLR2B Polyclonal Antibody (A5928)

Immunohistochemistry of paraffin-embedded mouse liver using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).

Inquire About This Product

Submit your question about A5928 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A5928? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order