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POLR2B Rabbit pAb (A5928)

Tested applications:WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - POLR2B Polyclonal Antibody (A5928)

Western blot analysis of extracts of various cell lines, using POLR2B antibody (A5928) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - POLR2B Polyclonal Antibody (A5928)

Immunohistochemistry of paraffin-embedded rat liver using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).

Overview

Product name	POLR2B Rabbit pAb
Catalog No.	A5928
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2B (NP_000929.1).
Sequence	MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATL
Gene ID	5431
Swiss prot	P30876
Synonyms	POLR2B; POL2RB; RPB2; hRPB140
Calculated MW	133kDa
Observed MW	134kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry
Positive samples	SH-SY5Y, A375, HepG2, BT-474, Mouse brain, Mouse spleen, Mouse ovary, Rat ovary
Cellular location	Nucleus
Customer validation	WB(Homo sapiens, Mus musculus)