Publications (4) Datasheet SDS
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of extracts of various cell lines, using POLR2B antibody (A5928) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat liver using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).
| Product name | POLR2B Rabbit pAb |
|---|---|
| Catalog No. | A5928 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2B (NP_000929.1). |
|---|---|
| Sequence | MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATL |
| Gene ID | 5431 |
| Swiss prot | P30876 |
| Synonyms | RPB2; POL2RB; hRPB140 |
| Calculated MW | 134kDa |
| Observed MW | 134kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us. |
| Application key | Western blotting Immunohistochemistry |
| Positive samples | SH-SY5Y, A375, HepG2, BT-474, Mouse brain, Mouse spleen, Mouse ovary, Rat ovary |
| Cellular location | Nucleus |
| Customer validation | WB(Homo sapiens, Mus musculus) ChIP-seq(X.tropicalis) |
Submit your question about A5928 below and we will get back to you within one business day.
Alternatively, call us at 888.754.5670.
Based on existing publications, the following genes are closely related to research on POLR2B. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to POLR2B. (Distance between topics and target gene indicate popularity.) POLR2B
* Data provided by citexs.com, for reference only.
Publishing research using A5928? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.
