Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

POLR2B Rabbit pAb (A5928)

Publications (4) Datasheet SDS

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - POLR2B Polyclonal Antibody (A5928)

Western blot analysis of extracts of various cell lines, using POLR2B antibody (A5928) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - POLR2B Polyclonal Antibody (A5928)

Immunohistochemistry of paraffin-embedded rat liver using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - POLR2B Polyclonal Antibody (A5928)

Immunohistochemistry of paraffin-embedded rat brain using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - POLR2B Polyclonal Antibody (A5928)

Immunohistochemistry of paraffin-embedded mouse liver using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).

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    Overview

    Product namePOLR2B Rabbit pAb
    Catalog No.A5928
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG

    Background

    This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants.

    Immunogen information

    ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2B (NP_000929.1).
    SequenceMYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATL
    Gene ID5431
    Swiss protP30876
    SynonymsRPB2; POL2RB; hRPB140
    Calculated MW134kDa
    Observed MW134kDa

    Applications

    ReactivityHuman, Mouse, Rat
    Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition
    Recommended dilution
    • WB 1:500 - 1:2000
    • IHC-P 1:50 - 1:200
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
    Application keyWestern blotting    Immunohistochemistry    
    Positive samplesSH-SY5Y, A375, HepG2, BT-474, Mouse brain, Mouse spleen, Mouse ovary, Rat ovary
    Cellular locationNucleus
    Customer validation

    WB(Homo sapiens, Mus musculus)

    ChIP-seq(X.tropicalis)

    Research Area

    POLR2B Rabbit pAb images

    ABclonal:Western blot - POLR2B Polyclonal Antibody (A5928) }

    Western blot - POLR2B Polyclonal Antibody (A5928)

    Western blot analysis of extracts of various cell lines, using POLR2B antibody (A5928) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - POLR2B Polyclonal Antibody (A5928) }

    Immunohistochemistry - POLR2B Polyclonal Antibody (A5928)

    Immunohistochemistry of paraffin-embedded rat liver using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - POLR2B Polyclonal Antibody (A5928) }

    Immunohistochemistry - POLR2B Polyclonal Antibody (A5928)

    Immunohistochemistry of paraffin-embedded rat brain using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - POLR2B Polyclonal Antibody (A5928) }

    Immunohistochemistry - POLR2B Polyclonal Antibody (A5928)

    Immunohistochemistry of paraffin-embedded mouse liver using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).

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    Related Targets

    Based on existing publications, the following genes are closely related to research on POLR2B. (Numbers indicate number of published articles where both targets appear.)

    1/

    Related Research Area

    Based on existing publications, the following research topics are related to POLR2B. (Distance between topics and target gene indicate popularity.) POLR2B

    * Data provided by citexs.com, for reference only.

    Publishing research using A5928? Please let us know so that we can cite the reference in this datasheet.

    * For research use only. Not for therapeutic or diagnostic purposes.

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