Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

POLR2B Rabbit pAb (A5928)

Publications (5) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - POLR2B Rabbit pAb (A5928)

Western blot analysis of extracts of various cell lines, using POLR2B antibody (A5928) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - POLR2B Rabbit pAb (A5928)

Immunohistochemistry analysis of paraffin-embedded rat liver using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - POLR2B Rabbit pAb (A5928)

Immunohistochemistry analysis of paraffin-embedded rat brain using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - POLR2B Rabbit pAb (A5928)

Immunohistochemistry analysis of paraffin-embedded mouse liver using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Overview

Product name POLR2B Rabbit pAb
Catalog No. A5928
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2B (NP_000929.1).
Sequence MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATL
Gene ID 5431
Swiss prot P30876
Synonyms RPB2; POL2RB; hRPB140; POLR2B
Calculated MW 134kDa
Observed MW 134kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples SH-SY5Y, A375, HepG2, BT-474, Mouse brain, Mouse spleen, Mouse ovary, Rat ovary
Cellular location Nucleus
Customer validation

WB (Homo sapiens, Mus musculus)

ChIP-seq (X.tropicalis)

ChIP-qPCR (Arabidopsis thaliana)

Documents

Certificate of Compliance

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Lot number

Research Area

POLR2B Rabbit pAb images

ABclonal:Western blot - POLR2B Rabbit pAb (A5928)}

Western blot - POLR2B Rabbit pAb (A5928)

Western blot analysis of extracts of various cell lines, using POLR2B antibody (A5928) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - POLR2B Rabbit pAb (A5928)}

Immunohistochemistry - POLR2B Rabbit pAb (A5928)

Immunohistochemistry analysis of paraffin-embedded rat liver using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - POLR2B Rabbit pAb (A5928)}

Immunohistochemistry - POLR2B Rabbit pAb (A5928)

Immunohistochemistry analysis of paraffin-embedded rat brain using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - POLR2B Rabbit pAb (A5928)}

Immunohistochemistry - POLR2B Rabbit pAb (A5928)

Immunohistochemistry analysis of paraffin-embedded mouse liver using POLR2B antibody (A5928) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on POLR2B. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to POLR2B. (Distance between topics and target gene indicate popularity.) POLR2B

* Data provided by citexs.com, for reference only.

Publishing research using A5928? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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