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PLSCR3 Polyclonal Antibody (A12371)

Datasheet

ABclonal:Western blot - PLSCR3 Polyclonal Antibody (A12371)
ABclonal:Immunofluorescence - PLSCR3 Polyclonal Antibody (A12371)

Overview

Product namePLSCR3 Polyclonal Antibody
Catalog No.A12371
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human PLSCR3 (NP_065093.2).
SequenceMAGYLPPKGYAPSPPPPYPVTPGYPEPALHPGPGQAPVPAQVPAPAPGFALFPSPGPVALGSAAPFLPLPGVPSGLEFLVQIDQILIHQKAERVETFLGWETCNRYELRSGAGQPLGQAAEESNCCARLCCGARRPLRVRLADPGDREVLRLLRPLHCGCSCCPCGLQEMEVQAPPGTTIGHVLQTWHPFLPKFSIQDADRQTVLRVVGPCWTCGCGTDTNFEVKTRDESRSVGRISKQWGGLVREALTDADDFGLQFPLDLDVRVKAVLLGATFLIDYMFFEKRGGAGPSAVTS
Gene ID57048
Swiss protQ9NRY6
SynonymsPLSCR3; phospholipid scramblase 3
Calculated MW31kDa
Observed MW32kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-549, 293T, HepG2, BxPC-3, HeLa, Mouse brain, Mouse heart, Mouse kidney
Cellular locationMitochondrion membrane, Single-pass type II membrane protein
ABclonal:Western blot - PLSCR3 Polyclonal Antibody (A12371) }

Western blot - PLSCR3 Polyclonal Antibody (A12371)

Western blot analysis of extracts of various cell lines, using PLSCR3 antibody (A12371) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - PLSCR3 Polyclonal Antibody (A12371) }

Immunofluorescence - PLSCR3 Polyclonal Antibody (A12371)

Immunofluorescence analysis of C6 cells using PLSCR3 Polyclonal Antibody (A12371) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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