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PLK1 Rabbit pAb (A2548)

Publication (1) Datasheet

ABclonal:Western blot - PLK1 Rabbit pAb (A2548)
ABclonal:Immunofluorescence - PLK1 Rabbit pAb (A2548)
ABclonal:Immunofluorescence - PLK1 Rabbit pAb (A2548)
ABclonal:Immunofluorescence - PLK1 Rabbit pAb (A2548)

Overview

Product namePLK1 Rabbit pAb
Catalog No.A2548
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 304-603 of human PLK1 (NP_005021.2).
SequenceFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS
Gene ID5347
Swiss protP53350
SynonymsPLK1; PLK; STPK13
Calculated MW68kDa
Observed MW65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, SW480, HT-29
Cellular locationChromosome, Cytoplasm, Midbody, Nucleus, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - PLK1 Rabbit pAb (A2548) }

Western blot - PLK1 Rabbit pAb (A2548)

Western blot analysis of extracts of various cell lines, using PLK1 antibody (A2548) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - PLK1 Rabbit pAb (A2548) }

Immunofluorescence - PLK1 Rabbit pAb (A2548)

Immunofluorescence analysis of C6 cells using PLK1 Rabbit pAb (A2548) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PLK1 Rabbit pAb (A2548) }

Immunofluorescence - PLK1 Rabbit pAb (A2548)

Immunofluorescence analysis of NIH-3T3 cells using PLK1 Rabbit pAb (A2548) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PLK1 Rabbit pAb (A2548) }

Immunofluorescence - PLK1 Rabbit pAb (A2548)

Immunofluorescence analysis of U-2 OS cells using PLK1 Rabbit pAb (A2548) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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