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PLCG1 Polyclonal Antibody (A15704)

Datasheet

ABclonal:Western blot - PLCG1 Polyclonal Antibody (A15704)
ABclonal:Immunofluorescence - PLCG1 Polyclonal Antibody (A15704)

Overview

Product namePLCG1 Polyclonal Antibody
Catalog No.A15704
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene catalyzes the formation of inositol 1, 4, 5-trisphosphate and diacylglycerol from phosphatidylinositol 4, 5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1202-1291 of human PLCG1 (NP_002651.2).
SequenceLASLLIKIDIFPAKQENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL
Gene ID5335
Swiss protP19174
SynonymsPLCG1; NCKAP3; PLC-II; PLC1; PLC148; PLCgamma1; phospholipase C gamma 1
Calculated MW148kDa
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, HepG2, 293T, Mouse lung, Rat brain
Cellular locationCell projection, lamellipodium, ruffle
ABclonal:Western blot - PLCG1 Polyclonal Antibody (A15704) }

Western blot - PLCG1 Polyclonal Antibody (A15704)

Western blot analysis of extracts of various cell lines, using PLCG1 antibody (A15704) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - PLCG1 Polyclonal Antibody (A15704) }

Immunofluorescence - PLCG1 Polyclonal Antibody (A15704)

Immunofluorescence analysis of U2OS cells using PLCG1 antibody (A15704) at dilution of 1:100. Blue: DAPI for nuclear staining.

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