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PKM2 Rabbit pAb (A0268)

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ABclonal:Western blot - PKM2 Rabbit pAb (A0268)

Western blot analysis of extracts of various cell lines, using PKM2 antibody (A0268) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - PKM2 Rabbit pAb (A0268)

Immunofluorescence analysis of U2OS cells using PKM2 antibody (A0268). Blue: DAPI for nuclear staining.

Review
ABclonal: review for PKM2 Rabbit pAb(A0268)
Review
ABclonal: review for PKM2 Rabbit pAb(A0268)
Review
ABclonal: review for PKM2 Rabbit pAb(A0268)
Review
ABclonal: review for PKM2 Rabbit pAb(A0268)

Overview

Product namePKM2 Rabbit pAb
Catalog No.A0268
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 11-221 of human PKM2 (NP_002645.3).
SequenceAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAV
Gene ID5315
Swiss protP14618
SynonymsCTHBP; HEL-S-30; OIP3; PK3; PKM2; TCB; THBP1; PKM
Calculated MW56kDa/57kDa/58kDa
Observed MW62kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesJurkat, HeLa, A-549
Cellular locationCytoplasm, Nucleus
Customer validation

(NCI-N87 cells, Human Bladder Cancer, Human cells, U251,U87MG)

IP(Homo sapiens)

WB(Homo sapiens)

IHC(Homo sapiens)

ABclonal:Western blot - PKM2 Rabbit pAb (A0268) }

Western blot - PKM2 Rabbit pAb (A0268)

Western blot analysis of extracts of various cell lines, using PKM2 antibody (A0268) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - PKM2 Rabbit pAb (A0268) }

Immunofluorescence - PKM2 Rabbit pAb (A0268)

Immunofluorescence analysis of U2OS cells using PKM2 antibody (A0268). Blue: DAPI for nuclear staining.

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