Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PINK1 Rabbit pAb (A11435)

Publications (15) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - PINK1 Rabbit pAb (A11435)

Western blot analysis of lysates from Rat liver, using PINK1 Rabbit pAb (A11435) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - PINK1 Rabbit pAb (A11435)

Western blot analysis of various lysates using PINK1 Rabbit pAb (A11435) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A11435)

Immunofluorescence analysis of A549 cells using PINK1 Rabbit pAb (A11435) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A11435)

Immunofluorescence analysis of NIH-3T3 cells using PINK1 Rabbit pAb (A11435) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A11435)

Immunofluorescence analysis of PC-12 cells using PINK1 Rabbit pAb (A11435) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A11435)

Immunofluorescence analysis of U-2 OS cells using PINK1 Rabbit pAb (A11435) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name PINK1 Rabbit pAb
Catalog No. A11435
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 143-511 of human PINK1 (NP_115785.1).
Sequence LDTRRLQGFRLEEYLIGQSIGKGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSL
Gene ID 65018
Swiss prot Q9BXM7
Synonyms BRPK; PARK6; PINK1
Calculated MW 63kDa
Observed MW 50kDa/63kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Mouse testis, Mouse heart, Rat liver
Cellular location Cytoplasm, Mitochondrion outer membrane, Single-pass membrane protein, cytosol
Customer validation

WB (Mus musculus, Homo sapiens)

IF (Mus musculus)

IHC (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

PINK1 Rabbit pAb images

ABclonal:Western blot - PINK1 Rabbit pAb (A11435)}

Western blot - PINK1 Rabbit pAb (A11435)

Western blot analysis of lysates from Rat liver, using PINK1 Rabbit pAb (A11435) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - PINK1 Rabbit pAb (A11435)}

Western blot - PINK1 Rabbit pAb (A11435)

Western blot analysis of various lysates using PINK1 Rabbit pAb (A11435) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A11435)}

Immunofluorescence - PINK1 Rabbit pAb (A11435)

Immunofluorescence analysis of A549 cells using PINK1 Rabbit pAb (A11435) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A11435)}

Immunofluorescence - PINK1 Rabbit pAb (A11435)

Immunofluorescence analysis of NIH-3T3 cells using PINK1 Rabbit pAb (A11435) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A11435)}

Immunofluorescence - PINK1 Rabbit pAb (A11435)

Immunofluorescence analysis of PC-12 cells using PINK1 Rabbit pAb (A11435) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A11435)}

Immunofluorescence - PINK1 Rabbit pAb (A11435)

Immunofluorescence analysis of U-2 OS cells using PINK1 Rabbit pAb (A11435) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on PINK1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PINK1. (Distance between topics and target gene indicate popularity.) PINK1

* Data provided by citexs.com, for reference only.

Publishing research using A11435? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

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Secondary Antibodies (23)

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