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PINK1 Rabbit pAb (A7131)

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ABclonal:Western blot - PINK1 Rabbit pAb (A7131)
ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A7131)
ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A7131)
Review
ABclonal: review for PINK1 Rabbit pAb(A7131)

Overview

Product namePINK1 Rabbit pAb
Catalog No.A7131
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 282-581 of human PINK1 (NP_115785.1).
SequenceTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL
Gene ID65018
Swiss protQ9BXM7
SynonymsPINK1; BRPK; PARK6
Calculated MW30kDa/62kDa
Observed MW46kDa, 60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesJurkat, mouse liver, rat heart
Cellular locationCytoplasm, Mitochondrion outer membrane, Single-pass membrane protein, cytosol
Customer validation

(U2OS,Saos-2)

WB(Homo sapiens)

ABclonal:Western blot - PINK1 Rabbit pAb (A7131) }

Western blot - PINK1 Rabbit pAb (A7131)

Western blot analysis of extracts of various cell lines, using PINK1 antibody (A7131) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A7131) }

Immunofluorescence - PINK1 Rabbit pAb (A7131)

Immunofluorescence analysis of NIH-3T3 cells using PINK1 Rabbit pAb (A7131) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A7131) }

Immunofluorescence - PINK1 Rabbit pAb (A7131)

Immunofluorescence analysis of U-2 OS cells using PINK1 Rabbit pAb (A7131) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

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