Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PINK1 Rabbit pAb (A7131)

Review (1) Publications (36) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - PINK1 Rabbit pAb (A7131)

Western blot analysis of lysates from Rat skeletal muscle, using PINK1 Rabbit pAb (A7131) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A7131)

Immunofluorescence analysis of NIH-3T3 cells using PINK1 Rabbit pAb (A7131) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A7131)

Immunofluorescence analysis of U-2 OS cells using PINK1 Rabbit pAb (A7131) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for PINK1 Rabbit pAb(A7131)

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Overview

Product name PINK1 Rabbit pAb
Catalog No. A7131
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 282-581 of human PINK1 (NP_115785.1).
Sequence TSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL
Gene ID 65018
Swiss prot Q9BXM7
Synonyms BRPK; PARK6; PINK1
Calculated MW 63kDa
Observed MW 60kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Rat skeletal muscle
Cellular location Cytoplasm, Mitochondrion outer membrane, Single-pass membrane protein, cytosol
Customer validation

WB (U2OS,Saos-2Homo sapiens, Mus musculus, Rattus norvegicus, Homo sapiens, Gallus gallus, Dianella ensifolia)

IF (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

PINK1 Rabbit pAb images

ABclonal:Western blot - PINK1 Rabbit pAb (A7131)}

Western blot - PINK1 Rabbit pAb (A7131)

Western blot analysis of lysates from Rat skeletal muscle, using PINK1 Rabbit pAb (A7131) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A7131)}

Immunofluorescence - PINK1 Rabbit pAb (A7131)

Immunofluorescence analysis of NIH-3T3 cells using PINK1 Rabbit pAb (A7131) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A7131)}

Immunofluorescence - PINK1 Rabbit pAb (A7131)

Immunofluorescence analysis of U-2 OS cells using PINK1 Rabbit pAb (A7131) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on PINK1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PINK1. (Distance between topics and target gene indicate popularity.) PINK1

* Data provided by citexs.com, for reference only.

Publishing research using A7131? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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