Products > Catalog Antibodies > Polyclonal Antibodies

PINK1 Rabbit pAb (A7131)

Review (1) Datasheet

ABclonal:Western blot - PINK1 Rabbit pAb (A7131)
ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A7131)
ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A7131)
Review
ABclonal: review for PINK1 Rabbit pAb(A7131)

Overview

Product namePINK1 Rabbit pAb
Catalog No.A7131
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 282-581 of human PINK1 (NP_115785.1).
SequenceTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL
Gene ID65018
Swiss protQ9BXM7
SynonymsPINK1; BRPK; PARK6
Calculated MW30kDa/62kDa
Observed MW46kDa, 60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesJurkat, mouse liver, rat heart
Cellular locationCytoplasm, Mitochondrion outer membrane, Single-pass membrane protein, cytosol
Customer validation

(U2OS,Saos-2)

ABclonal:Western blot - PINK1 Rabbit pAb (A7131) }

Western blot - PINK1 Rabbit pAb (A7131)

Western blot analysis of extracts of various cell lines, using PINK1 antibody (A7131) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A7131) }

Immunofluorescence - PINK1 Rabbit pAb (A7131)

Immunofluorescence analysis of NIH-3T3 cells using PINK1 Rabbit pAb (A7131) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PINK1 Rabbit pAb (A7131) }

Immunofluorescence - PINK1 Rabbit pAb (A7131)

Immunofluorescence analysis of U-2 OS cells using PINK1 Rabbit pAb (A7131) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Submit an review for A7131

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A7131 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
5 μL sample size available Add to Cart
Contact us to order