Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

PI3 Kinase p85 beta Mouse mAb (A5241)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - PI3 Kinase p85 beta Mouse mAb (A5241)

Western blot analysis of various lysates using PI3 Kinase p85 beta Mouse mAb (A5241) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name PI3 Kinase p85 beta Mouse mAb
Catalog No. A5241
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Three transcript variants, one protein coding and the other two non-protein coding, have been found for this gene.

Immunogen information

Immunogen Recombinant protein of human PI3 Kinase p85 beta
Sequence Email for sequence
Gene ID 5296
Swiss prot O00459
Synonyms p85; MPPH; P85B; MPPH1; p85beta; p85-BETA; PI3 Kinase p85 beta
Calculated MW 82kDa
Observed MW 100kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples K-562, MCF7, HeLa, SH-SY5Y, SW620, Jurkat, Mouse brain, Rat brain
Cellular location cytosol, focal adhesion, nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

PI3 Kinase p85 beta Mouse mAb images

ABclonal:Western blot - PI3 Kinase p85 beta Mouse mAb (A5241)}

Western blot - PI3 Kinase p85 beta Mouse mAb (A5241)

Western blot analysis of various lysates using PI3 Kinase p85 beta Mouse mAb (A5241) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on PIK3R2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PIK3R2. (Distance between topics and target gene indicate popularity.) PIK3R2

* Data provided by citexs.com, for reference only.

Publishing research using A5241? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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