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PI3 Kinase p110 beta Rabbit mAb (A11906)

Datasheet

ABclonal:Western blot - PI3 Kinase p110 beta Monoclonal Antibody (A11906)

Overview

Product namePI3 Kinase p110 beta Rabbit mAb
Catalog No.A11906
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant protein of human PI3 Kinase p110 beta
SequenceEmail for sequence
Gene ID5291
Swiss protP42338
SynonymsPIK3CB; P110BETA; PI3K; PI3KBETA; PIK3C1
Calculated MW122kDa
Observed MW123kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
ICC 1:50 - 1:200
IF 1:50 - 1:100
FC 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    immunocytochemistry    Immunofluorescence    Flow Cytometry    
Positive samplesRat brain, Mouse brain
Cellular locationCytoplasm, Nucleus
ABclonal:Western blot - PI3 Kinase p110 beta Monoclonal Antibody (A11906) }

Western blot - PI3 Kinase p110 beta Monoclonal Antibody (A11906)

Western blot analysis of extracts of various cell lines, using PI3 Kinase p110 beta antibody (A11906).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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