Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PIK3CA Rabbit pAb (A16950)

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - PIK3CA Rabbit pAb (A16950)

Western blot analysis of extracts of 293T cells, using PIK3CA antibody (A16950) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Overview

Product name	PIK3CA Rabbit pAb
Catalog No.	A16950
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4, 5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 401-600 of human PIK3CA (NP_006209.2).
Sequence	RLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLKAISTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAME
Gene ID	5290
Swiss prot	P42336
Synonyms	MCM; CCM4; CWS5; MCAP; PI3K; CLAPO; CLOVE; MCMTC; PI3K-alpha; p110-alpha; PIK3CA
Calculated MW	124kDa
Observed MW	110kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key	Western blotting
Positive samples	293T
Cellular location	cytoplasm, cytosol, intercalated disc, perinuclear region of cytoplasm, plasma membrane
Customer validation	WB (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

PIK3CA Rabbit pAb images

Western blot - PIK3CA Rabbit pAb (A16950)

Product Protocols

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Related Targets

Based on existing publications, the following genes are closely related to research on PIK3CA. (Numbers indicate number of published articles where both targets appear.)

◀1/▶

Related Research Area

Based on existing publications, the following research topics are related to PIK3CA. (Distance between topics and target gene indicate popularity.) PIK3CA

* Data provided by citexs.com, for reference only.