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PIK3CA Rabbit pAb (A12484)

Publication (1) Datasheet

ABclonal:Western blot - PIK3CA Rabbit pAb (A12484)

Western blot analysis of extracts of various cell lines, using PIK3CA antibody (A12484) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - PIK3CA Rabbit pAb (A12484)

Western blot analysis of extracts of 293T cells, using PIK3CA antibody (A12484) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A12484)

Immunohistochemistry of paraffin-embedded human breast cancer using PIK3CA Rabbit pAb (A12484) at dilution of 1:150 (40x lens).

ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A12484)

Immunohistochemistry of paraffin-embedded mouse kidney using PIK3CA Rabbit pAb (A12484) at dilution of 1:150 (40x lens).

ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A12484)

Immunohistochemistry of paraffin-embedded rat liver using PIK3CA Rabbit pAb (A12484) at dilution of 1:150 (40x lens).

Overview

Product namePIK3CA Rabbit pAb
Catalog No.A12484
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4, 5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22.
ImmunogenA synthetic peptide of human PIK3CA
SequenceEmail for sequence
Gene ID5290
Swiss protP42336
SynonymsPIK3CA; CLOVE; CWS5; MCAP; MCM; MCMTC; PI3K; PI3K-alpha
Calculated MW124kDa
Observed MW110KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples293T, Mouse brain, Rat brain
Cellular location
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - PIK3CA Rabbit pAb (A12484) }

Western blot - PIK3CA Rabbit pAb (A12484)

Western blot analysis of extracts of various cell lines, using PIK3CA antibody (A12484) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - PIK3CA Rabbit pAb (A12484) }

Western blot - PIK3CA Rabbit pAb (A12484)

Western blot analysis of extracts of 293T cells, using PIK3CA antibody (A12484) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A12484) }

Immunohistochemistry - PIK3CA Rabbit pAb (A12484)

Immunohistochemistry of paraffin-embedded human breast cancer using PIK3CA Rabbit pAb (A12484) at dilution of 1:150 (40x lens).
ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A12484) }

Immunohistochemistry - PIK3CA Rabbit pAb (A12484)

Immunohistochemistry of paraffin-embedded mouse kidney using PIK3CA Rabbit pAb (A12484) at dilution of 1:150 (40x lens).
ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A12484) }

Immunohistochemistry - PIK3CA Rabbit pAb (A12484)

Immunohistochemistry of paraffin-embedded rat liver using PIK3CA Rabbit pAb (A12484) at dilution of 1:150 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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