Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PIK3CA Rabbit pAb (A12484)

Publications (3) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - PIK3CA Rabbit pAb (A12484)

Western blot analysis of extracts of various cell lines, using PIK3CA antibody (A12484) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - PIK3CA Rabbit pAb (A12484)

Western blot analysis of extracts of 293T cells, using PIK3CA antibody (A12484) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A12484)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using PIK3CA Rabbit pAb (A12484) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A12484)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using PIK3CA Rabbit pAb (A12484) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A12484)

Immunohistochemistry analysis of paraffin-embedded rat liver using PIK3CA Rabbit pAb (A12484) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

You may also interested in:

Overview

Product name PIK3CA Rabbit pAb
Catalog No. A12484
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4, 5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human PIK3CA (NP_006209.2).
Sequence DCFTMPSYSRRISTATPYMNGETSTKSLWVINSALRIKILCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLNYDIYIPDLPRAARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLKAISTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAME
Gene ID 5290
Swiss prot P42336
Synonyms MCM; CCM4; CWS5; MCAP; PI3K; CLAPO; CLOVE; MCMTC; PI3K-alpha; p110-alpha; PIK3CA
Calculated MW 124kDa
Observed MW 110kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples 293T, Mouse brain, Rat brain
Cellular location cytoplasm, cytosol, intercalated disc, perinuclear region of cytoplasm, plasma membrane
Customer validation

WB (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

PIK3CA Rabbit pAb images

ABclonal:Western blot - PIK3CA Rabbit pAb (A12484)}

Western blot - PIK3CA Rabbit pAb (A12484)

Western blot analysis of extracts of various cell lines, using PIK3CA antibody (A12484) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - PIK3CA Rabbit pAb (A12484)}

Western blot - PIK3CA Rabbit pAb (A12484)

Western blot analysis of extracts of 293T cells, using PIK3CA antibody (A12484) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A12484)}

Immunohistochemistry - PIK3CA Rabbit pAb (A12484)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using PIK3CA Rabbit pAb (A12484) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A12484)}

Immunohistochemistry - PIK3CA Rabbit pAb (A12484)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using PIK3CA Rabbit pAb (A12484) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A12484)}

Immunohistochemistry - PIK3CA Rabbit pAb (A12484)

Immunohistochemistry analysis of paraffin-embedded rat liver using PIK3CA Rabbit pAb (A12484) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Inquire About This Product

Submit your question about A12484 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on PIK3CA. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PIK3CA. (Distance between topics and target gene indicate popularity.) PIK3CA

* Data provided by citexs.com, for reference only.

Publishing research using A12484? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order