Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PIK3CA Rabbit pAb (A0265)

Publications (69) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - PIK3CA Rabbit pAb (A0265)

Western blot analysis of various lysates, using PIK3CA Rabbit pAb (A0265) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A0265)

Immunohistochemistry analysis of PIK3CA in paraffin-embedded mouse kidney using PIK3CA Rabbit pAb (A0265) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A0265)

Immunohistochemistry analysis of PIK3CA in paraffin-embedded rat liver using PIK3CA Rabbit pAb (A0265) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PIK3CA Rabbit pAb (A0265)

Immunofluorescence analysis of NIH/3T3 cells using PIK3CA Rabbit pAb (A0265) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PIK3CA Rabbit pAb (A0265)

Immunofluorescence analysis of PC-12 cells using PIK3CA Rabbit pAb (A0265) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PIK3CA Rabbit pAb (A0265)

Immunofluorescence analysis of U2OS cells using PIK3CA Rabbit pAb (A0265) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name PIK3CA Rabbit pAb
Catalog No. A0265
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4, 5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 401-600 of human PIK3CA (NP_006209.2).
Sequence RLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLKAISTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAME
Gene ID 5290
Swiss prot P42336
Synonyms MCM; CCM4; CWS5; MCAP; PI3K; CLAPO; CLOVE; MCMTC; PI3K-alpha; p110-alpha; PIK3CA
Calculated MW 124kDa
Observed MW 110kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples Jurkat, MCF7, Mouse brain
Cellular location cytoplasm, cytosol, intercalated disc, perinuclear region of cytoplasm, plasma membrane
Customer validation

WB (Mus musculus, Homo sapiens, Rattus norvegicus, Cyprinus carpio, Capra hircus, Gallus gallus, Ctenopharyngodon idellus, Sus scrofa)

IHC (Mus musculus, Homo sapiens, Rattus norvegicus)

IF (Rattus norvegicus, Homo sapiens, Mus musculus)

ICH (Rattus norvegicus)

Co-IP (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

PIK3CA Rabbit pAb images

ABclonal:Western blot - PIK3CA Rabbit pAb (A0265)}

Western blot - PIK3CA Rabbit pAb (A0265)

Western blot analysis of various lysates, using PIK3CA Rabbit pAb (A0265) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A0265)}

Immunohistochemistry - PIK3CA Rabbit pAb (A0265)

Immunohistochemistry analysis of PIK3CA in paraffin-embedded mouse kidney using PIK3CA Rabbit pAb (A0265) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A0265)}

Immunohistochemistry - PIK3CA Rabbit pAb (A0265)

Immunohistochemistry analysis of PIK3CA in paraffin-embedded rat liver using PIK3CA Rabbit pAb (A0265) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - PIK3CA Rabbit pAb (A0265)}

Immunofluorescence - PIK3CA Rabbit pAb (A0265)

Immunofluorescence analysis of NIH/3T3 cells using PIK3CA Rabbit pAb (A0265) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PIK3CA Rabbit pAb (A0265)}

Immunofluorescence - PIK3CA Rabbit pAb (A0265)

Immunofluorescence analysis of PC-12 cells using PIK3CA Rabbit pAb (A0265) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PIK3CA Rabbit pAb (A0265)}

Immunofluorescence - PIK3CA Rabbit pAb (A0265)

Immunofluorescence analysis of U2OS cells using PIK3CA Rabbit pAb (A0265) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on PIK3CA. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PIK3CA. (Distance between topics and target gene indicate popularity.) PIK3CA

* Data provided by citexs.com, for reference only.

Publishing research using A0265? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

ELISA Kits (1)

Secondary Antibodies (22)

* For research use only. Not for therapeutic or diagnostic purposes.

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