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PIK3CA Rabbit pAb (A0265)

Publications (13) Datasheet

ABclonal:Western blot - PIK3CA Rabbit pAb (A0265)

Western blot analysis of extracts of various cell lines, using PIK3CA antibody (A0265) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A0265)

Immunohistochemistry of paraffin-embedded rat kidney using PIK3CA antibody (A0265) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A0265)

Immunohistochemistry of paraffin-embedded human colon carcinoma using PIK3CA antibody (A0265) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A0265)

Immunohistochemistry of paraffin-embedded mouse kidney using PIK3CA antibody (A0265) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - PIK3CA Rabbit pAb (A0265)

Immunofluorescence analysis of HeLa cells using PIK3CA antibody (A0265) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PIK3CA Rabbit pAb (A0265)

Immunofluorescence analysis of NIH/3T3 cells using PIK3CA antibody (A0265) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product namePIK3CA Rabbit pAb
Catalog No.A0265
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4, 5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 401-600 of human PIK3CA (NP_006209.2).
SequenceRLCLSICSVKGRKGAKEEHCPLAWGNINLFDYTDTLVSGKMALNLWPVPHGLEDLLNPIGVTGSNPNKETPCLELEFDWFSSVVKFPDMSVIEEHANWSVSREAGFSYSHAGLSNRLARDNELRENDKEQLKAISTRDPLSEITEQEKDFLWSHRHYCVTIPEILPKLLLSVKWNSRDEVAQMYCLVKDWPPIKPEQAME
Gene ID5290
Swiss protP42336
SynonymsPIK3CA; CLOVE; CWS5; MCAP; MCM; MCMTC; PI3K; PI3K-alpha
Calculated MW124kDa
Observed MW110KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-549, HeLa, Mouse lung, Mouse spleen
Cellular location
Customer validation

WB(Homo sapiens, Cyprinus carpio, Capra hircus, Gallus gallus, Ctenopharyngodon idellus, Mus musculus, Rattus norvegicus)

Co-IP(Homo sapiens)

ABclonal:Western blot - PIK3CA Rabbit pAb (A0265) }

Western blot - PIK3CA Rabbit pAb (A0265)

Western blot analysis of extracts of various cell lines, using PIK3CA antibody (A0265) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A0265) }

Immunohistochemistry - PIK3CA Rabbit pAb (A0265)

Immunohistochemistry of paraffin-embedded rat kidney using PIK3CA antibody (A0265) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A0265) }

Immunohistochemistry - PIK3CA Rabbit pAb (A0265)

Immunohistochemistry of paraffin-embedded human colon carcinoma using PIK3CA antibody (A0265) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PIK3CA Rabbit pAb (A0265) }

Immunohistochemistry - PIK3CA Rabbit pAb (A0265)

Immunohistochemistry of paraffin-embedded mouse kidney using PIK3CA antibody (A0265) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - PIK3CA Rabbit pAb (A0265) }

Immunofluorescence - PIK3CA Rabbit pAb (A0265)

Immunofluorescence analysis of HeLa cells using PIK3CA antibody (A0265) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PIK3CA Rabbit pAb (A0265) }

Immunofluorescence - PIK3CA Rabbit pAb (A0265)

Immunofluorescence analysis of NIH/3T3 cells using PIK3CA antibody (A0265) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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