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PIK3C3/VPS34 Rabbit pAb (A12483)

Publications (2) Datasheet

ABclonal:Western blot - PIK3C3/VPS34 Rabbit pAb (A12483)

Western blot analysis of extracts of various cell lines, using PIK3C3/VPS34/VPS34 antibody (A12483) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product namePIK3C3/VPS34 Rabbit pAb
Catalog No.A12483
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 150-250 of human PIK3C3/VPS34/VPS34 (NP_002638.2).
SequenceEADGSEPTKTPGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEKRSSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSF
Gene ID5289
Swiss protQ8NEB9
SynonymsPIK3C3; VPS34; Vps34; hVps34
Calculated MW101kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesSH-SY5Y, Mouse brain, Rat brain
Cellular locationCytoplasmic vesicle, Late endosome, Midbody, autophagosome
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - PIK3C3/VPS34 Rabbit pAb (A12483) }

Western blot - PIK3C3/VPS34 Rabbit pAb (A12483)

Western blot analysis of extracts of various cell lines, using PIK3C3/VPS34/VPS34 antibody (A12483) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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