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PIK3C3/VPS34 Rabbit pAb (A12483)

Publications (2) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - PIK3C3/VPS34 Rabbit pAb (A12483)

Western blot analysis of extracts of various cell lines, using PIK3C3/VPS34 antibody (A12483) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - PIK3C3/VPS34 Rabbit pAb (A12483)

Immunofluorescence analysis of NIH/3T3 cells using PIK3C3/VPS34 Rabbit pAb (A12483) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product namePIK3C3/VPS34 Rabbit pAb
Catalog No.A12483
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. As part of PI3KC3-C1, promotes endoplasmic reticulum membrane curvature formation prior to vesicle budding. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 130-412 of human PIK3C3/VPS34 (NP_002638.2).
SequenceFGKYGMFRQGMHDLKVWPNVEADGSEPTKTPGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEKRSSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVKVPDPQMSMENLVESKHHKLARSLRSGPSDHDLKPNAATRDQLNIIVSYPPTKQLTYEEQDLVWKFRYYLTNQEKALTKFLKCVNWDLPQEAKQALELLGKWKPMDVEDSLELLSSHYTNPTVRRYAVARLRQADDEDLLMYLLQLVQALKYENFDDIK
Gene ID5289
Swiss protQ8NEB9
SynonymsPIK3C3; VPS34; Vps34; hVps34
Calculated MW101kDa
Observed MW100KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:100 - 1:200
  • IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, C6, Mouse lung, Mouse brain, Rat brain
Cellular locationCytoplasmic vesicle, Late endosome, Midbody, autophagosome
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - PIK3C3/VPS34 Rabbit pAb (A12483)}

Western blot - PIK3C3/VPS34 Rabbit pAb (A12483)

Western blot analysis of extracts of various cell lines, using PIK3C3/VPS34 antibody (A12483) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - PIK3C3/VPS34 Rabbit pAb (A12483)}

Immunofluorescence - PIK3C3/VPS34 Rabbit pAb (A12483)

Immunofluorescence analysis of NIH/3T3 cells using PIK3C3/VPS34 Rabbit pAb (A12483) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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