Publications (2) Datasheet SDS COA
Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Reactivity:Mouse, Rat
Western blot analysis of various lysates using PIK3C3/VPS34 Rabbit mAb (A12295) at 1：1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunofluorescence analysis of NIH-3T3 cells using PIK3C3/VPS34 Rabbit mAb (A12295) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
| Product name | PIK3C3/VPS34 Rabbit mAb |
|---|---|
| Catalog No. | A12295 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC0286 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 788-887 of human PIK3C3/VPS34 (Q8NEB9). |
|---|---|
| Sequence | GGTQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKKVQDKFRLDLSDEEAVHYMQSLIDESVHALFAAVVEQIHKFAQYWRK |
| Gene ID | 5289 |
| Swiss prot | Q8NEB9 |
| Synonyms | VPS34; Vps34; hVps34; PIK3C3/VPS34 |
| Calculated MW | 102kDa |
| Observed MW | 100kDa |
| Reactivity | Mouse, Rat |
|---|---|
| Tested applications | Testing results |
| IF/ICC | |
| WB | |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunofluorescence Immunoprecipitation |
| Positive samples | RD, Mouse brain, Rat brain |
| Cellular location | Cytoplasmic vesicle, Late endosome, Midbody, autophagosome |
| Customer validation | WB (Homo sapiens) IHC (Rattus norvegicus) |
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Based on existing publications, the following genes are closely related to research on PIK3C3. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to PIK3C3. (Distance between topics and target gene indicate popularity.) PIK3C3
* Data provided by citexs.com, for reference only.
Publishing research using A12295? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.
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