Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PI3 Kinase p85 alpha Rabbit pAb (A11402)

Publications (2) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Mouse, Rat

ABclonal:Western blot - PI3 Kinase p85 alpha Rabbit pAb (A11402)

Western blot analysis of various lysates using PI3 Kinase p85 alpha Rabbit pAb (A11402) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - PI3 Kinase p85 alpha Rabbit pAb (A11402)

Immunofluorescence analysis of PC-12 cells using PI3 Kinase p85 alpha Rabbit pAb (A11402) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PI3 Kinase p85 alpha Rabbit pAb (A11402)

Immunofluorescence analysis of NIH/3T3 cells using PI3 Kinase p85 alpha Rabbit pAb (A11402) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name PI3 Kinase p85 alpha Rabbit pAb
Catalog No. A11402
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 240-380 of human PI3 Kinase p85 alpha (NP_852556.2).
Sequence EKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRE
Gene ID 5295
Swiss prot P27986
Synonyms p85; AGM7; GRB1; IMD36; p85alpha; p85-ALPHA; PI3 Kinase p85 alpha
Calculated MW 84kDa
Observed MW 85kDa

Applications

Reactivity Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Mouse brain, Mouse liver, Rat brain, Rat liver
Cellular location cell-cell junction, cis-Golgi network, cytoplasm, cytosol, nucleus, perinuclear region of cytoplasm, plasma membrane
Customer validation

WB (Mus musculus, Gallus gallus)

Documents

Certificate of Compliance

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Lot number

Research Area

PI3 Kinase p85 alpha Rabbit pAb images

ABclonal:Western blot - PI3 Kinase p85 alpha Rabbit pAb (A11402)}

Western blot - PI3 Kinase p85 alpha Rabbit pAb (A11402)

Western blot analysis of various lysates using PI3 Kinase p85 alpha Rabbit pAb (A11402) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - PI3 Kinase p85 alpha Rabbit pAb (A11402)}

Immunofluorescence - PI3 Kinase p85 alpha Rabbit pAb (A11402)

Immunofluorescence analysis of PC-12 cells using PI3 Kinase p85 alpha Rabbit pAb (A11402) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PI3 Kinase p85 alpha Rabbit pAb (A11402)}

Immunofluorescence - PI3 Kinase p85 alpha Rabbit pAb (A11402)

Immunofluorescence analysis of NIH/3T3 cells using PI3 Kinase p85 alpha Rabbit pAb (A11402) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on PIK3R1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PIK3R1. (Distance between topics and target gene indicate popularity.) PIK3R1

* Data provided by citexs.com, for reference only.

Publishing research using A11402? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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