Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

PI3 Kinase p85 alpha Rabbit mAb (A4992)

Publications (19) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - PI3 Kinase p85 alpha Rabbit mAb (A4992)

Western blot analysis of various lysates, using PI3 Kinase p85 alpha Rabbit mAb (A4992) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - PI3 Kinase p85 alpha Rabbit mAb (A4992)

Immunohistochemistry analysis of paraffin-embedded mouse brain using PI3 Kinase p85 alpha Rabbit mAb (A4992) at dilution of 1:60 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PI3 Kinase p85 alpha Rabbit mAb (A4992)

Immunohistochemistry analysis of paraffin-embedded rat brain using PI3 Kinase p85 alpha Rabbit mAb (A4992) at dilution of 1:60 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Overview

Product name PI3 Kinase p85 alpha Rabbit mAb
Catalog No. A4992
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC55272

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PI3 Kinase p85 alpha (P27986).
Sequence MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEARPEEIGWLNGYNETTGERGDFPGTYVEYIGRKKISPPTPKPRPPRPLPVAPG
Gene ID 5295
Swiss prot P27986
Synonyms p85; AGM7; GRB1; IMD36; p85alpha; p85-ALPHA; PI3 Kinase p85 alpha
Calculated MW 84kDa
Observed MW 85kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
WB HumanMouseRat
IHC-P MouseRat
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Jurkat, 293F, NIH/3T3, Rat lung
Cellular location cell-cell junction, cis-Golgi network, cytoplasm, cytosol, nucleus, perinuclear region of cytoplasm, plasma membrane
Customer validation

WB (Homo sapiens, Mus musculus, Rattus norvegicus, mice, Gallus gallus, gallus domesticus)

IP (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

PI3 Kinase p85 alpha Rabbit mAb images

ABclonal:Western blot - PI3 Kinase p85 alpha Rabbit mAb (A4992)}

Western blot - PI3 Kinase p85 alpha Rabbit mAb (A4992)

Western blot analysis of various lysates, using PI3 Kinase p85 alpha Rabbit mAb (A4992) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - PI3 Kinase p85 alpha Rabbit mAb (A4992)}

Immunohistochemistry - PI3 Kinase p85 alpha Rabbit mAb (A4992)

Immunohistochemistry analysis of paraffin-embedded mouse brain using PI3 Kinase p85 alpha Rabbit mAb (A4992) at dilution of 1:60 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PI3 Kinase p85 alpha Rabbit mAb (A4992)}

Immunohistochemistry - PI3 Kinase p85 alpha Rabbit mAb (A4992)

Immunohistochemistry analysis of paraffin-embedded rat brain using PI3 Kinase p85 alpha Rabbit mAb (A4992) at dilution of 1:60 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on PIK3R1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PIK3R1. (Distance between topics and target gene indicate popularity.) PIK3R1

* Data provided by citexs.com, for reference only.

Publishing research using A4992? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Secondary Antibodies (22)

* For research use only. Not for therapeutic or diagnostic purposes.

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