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PI3 Kinase p110 beta Rabbit pAb (A0982)

Publications (2) Datasheet

ABclonal:Western blot - PI3 Kinase p110 beta Rabbit pAb (A0982)

Western blot analysis of extracts of various cell lines, using PI3 Kinase p110 beta Rabbit pAb (A0982) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - PI3 Kinase p110 beta Rabbit pAb (A0982)

Immunohistochemistry of paraffin-embedded mouse lung using PI3 Kinase p110 beta antibody (A0982) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - PI3 Kinase p110 beta Rabbit pAb (A0982)

Immunohistochemistry of paraffin-embedded human lung using PI3 Kinase p110 beta antibody (A0982) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - PI3 Kinase p110 beta Rabbit pAb (A0982)

Immunohistochemistry of paraffin-embedded human colon carcinoma using PI3 Kinase p110 beta antibody (A0982) at dilution of 1:100 (40x lens).

Overview

Product namePI3 Kinase p110 beta Rabbit pAb
Catalog No.A0982
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 771-1070 of human PI3 Kinase p110 beta (NP_006210.1).
SequenceLYVEKCKYMDSKMKPLWLVYNNKVFGEDSVGVIFKNGDDLRQDMLTLQMLRLMDLLWKEAGLDLRMLPYGCLATGDRSGLIEVVSTSETIADIQLNSSNVAAAAAFNKDALLNWLKEYNSGDDLDRAIEEFTLSCAGYCVASYVLGIGDRHSDNIMVKKTGQLFHIDFGHILGNFKSKFGIKRERVPFILTYDFIHVIQQGKTGNTEKFGRFRQCCEDAYLILRRHGNLFITLFALMLTAGLPELTSVKDIQYLKDSLALGKSEEEALKQFKQKFDEALRESWTTKVNWMAHTVRKDYRS
Gene ID5291
Swiss protP42338
SynonymsPIK3CB; P110BETA; PI3K; PI3KBETA; PIK3C1
Calculated MW122kDa
Observed MW123KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7, Mouse brain
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - PI3 Kinase p110 beta Rabbit pAb (A0982) }

Western blot - PI3 Kinase p110 beta Rabbit pAb (A0982)

Western blot analysis of extracts of various cell lines, using PI3 Kinase p110 beta Rabbit pAb (A0982) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - PI3 Kinase p110 beta Rabbit pAb (A0982) }

Immunohistochemistry - PI3 Kinase p110 beta Rabbit pAb (A0982)

Immunohistochemistry of paraffin-embedded mouse lung using PI3 Kinase p110 beta antibody (A0982) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PI3 Kinase p110 beta Rabbit pAb (A0982) }

Immunohistochemistry - PI3 Kinase p110 beta Rabbit pAb (A0982)

Immunohistochemistry of paraffin-embedded human lung using PI3 Kinase p110 beta antibody (A0982) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PI3 Kinase p110 beta Rabbit pAb (A0982) }

Immunohistochemistry - PI3 Kinase p110 beta Rabbit pAb (A0982)

Immunohistochemistry of paraffin-embedded human colon carcinoma using PI3 Kinase p110 beta antibody (A0982) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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