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PGP9.5/UCHL1 Rabbit mAb (A19101)

Publication (1) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - PGP9.5/UCHL1 Rabbit mAb (A19101)

Western blot analysis of extracts of various cell lines, using PGP9.5/UCHL1/UCHL1 antibody (A19101) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - PGP9.5/UCHL1 Rabbit mAb (A19101)

Immunohistochemistry of paraffin-embedded mouse brain using PGP9.5/UCHL1/UCHL1 Rabbit mAb (A19101) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PGP9.5/UCHL1 Rabbit mAb (A19101)

Immunohistochemistry of paraffin-embedded rat brain using PGP9.5/UCHL1/UCHL1 Rabbit mAb (A19101) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PGP9.5/UCHL1 Rabbit mAb (A19101)

Immunohistochemistry of paraffin-embedded human oophoroma using PGP9.5/UCHL1/UCHL1 Rabbit mAb (A19101) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PGP9.5/UCHL1 Rabbit mAb (A19101)

Immunofluorescence analysis of mouse brain using PGP9.5/UCHL1/UCHL1 Rabbit mAb (A19101) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product namePGP9.5/UCHL1 Rabbit mAb
Catalog No.A19101
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]
ImmunogenA synthesized peptide derived from human PGP9.5/UCHL1/UCHL1
SequenceEmail for sequence
Gene ID7345
Swiss protP09936
SynonymsHEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; SPG79; Uch-L1; UCHL1
Calculated MW25kDa
Observed MW27kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, DU145, SH-SY5Y, A-549, Mouse brain, Rat brain
Cellular locationCytoplasm, Endoplasmic reticulum membrane, Lipid-anchor
Customer validation

IF(Mus musculus)

ABclonal:Western blot - PGP9.5/UCHL1 Rabbit mAb (A19101) }

Western blot - PGP9.5/UCHL1 Rabbit mAb (A19101)

Western blot analysis of extracts of various cell lines, using PGP9.5/UCHL1/UCHL1 antibody (A19101) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - PGP9.5/UCHL1 Rabbit mAb (A19101) }

Immunohistochemistry - PGP9.5/UCHL1 Rabbit mAb (A19101)

Immunohistochemistry of paraffin-embedded mouse brain using PGP9.5/UCHL1/UCHL1 Rabbit mAb (A19101) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PGP9.5/UCHL1 Rabbit mAb (A19101) }

Immunohistochemistry - PGP9.5/UCHL1 Rabbit mAb (A19101)

Immunohistochemistry of paraffin-embedded rat brain using PGP9.5/UCHL1/UCHL1 Rabbit mAb (A19101) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PGP9.5/UCHL1 Rabbit mAb (A19101) }

Immunohistochemistry - PGP9.5/UCHL1 Rabbit mAb (A19101)

Immunohistochemistry of paraffin-embedded human oophoroma using PGP9.5/UCHL1/UCHL1 Rabbit mAb (A19101) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - PGP9.5/UCHL1 Rabbit mAb (A19101) }

Immunofluorescence - PGP9.5/UCHL1 Rabbit mAb (A19101)

Immunofluorescence analysis of mouse brain using PGP9.5/UCHL1/UCHL1 Rabbit mAb (A19101) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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