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PGP9.5/UCHL1 Rabbit mAb (A19101)

Tested applications:WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat

Western blot analysis of extracts of various cell lines, using PGP9.5/UCHL1/UCHL1 antibody (A19101) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - PGP9.5/UCHL1 Rabbit mAb (A19101)

Immunohistochemistry of paraffin-embedded mouse brain using PGP9.5/UCHL1/UCHL1 Rabbit mAb (A19101) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded rat brain using PGP9.5/UCHL1/UCHL1 Rabbit mAb (A19101) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human oophoroma using PGP9.5/UCHL1/UCHL1 Rabbit mAb (A19101) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PGP9.5/UCHL1 Rabbit mAb (A19101)

Immunofluorescence analysis of mouse brain using PGP9.5/UCHL1/UCHL1 Rabbit mAb (A19101) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	PGP9.5/UCHL1 Rabbit mAb
Catalog No.	A19101
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]

Immunogen information

Immunogen	A synthesized peptide derived from human PGP9.5/UCHL1/UCHL1
Sequence	Email for sequence
Gene ID	7345
Swiss prot	P09936
Synonyms	HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; SPG79; Uch-L1; UCHL1
Calculated MW	25kDa
Observed MW	27kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	293T, DU145, SH-SY5Y, A-549, Mouse brain, Rat brain
Cellular location	Cytoplasm, Endoplasmic reticulum membrane, Lipid-anchor
Customer validation	IF(Mus musculus)

Research Area

PGP9.5/UCHL1 Rabbit mAb images

Western blot - PGP9.5/UCHL1 Rabbit mAb (A19101)

Immunohistochemistry - PGP9.5/UCHL1 Rabbit mAb (A19101)

Immunofluorescence - PGP9.5/UCHL1 Rabbit mAb (A19101)

Immunofluorescence analysis of mouse brain using PGP9.5/UCHL1/UCHL1 Rabbit mAb (A19101) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.