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PGC1α Rabbit pAb (A12348)

Reviews (2)Publications (18) Datasheet

ABclonal:Western blot - PGC1α Rabbit pAb (A12348)

Western blot analysis of extracts of various cell lines, using PGC1α antibody (A12348) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - PGC1α Rabbit pAb (A12348)

Immunohistochemistry of paraffin-embedded mouse heart using PGC1α Rabbit pAb (A12348) at dilution of 1:50 (40x lens).

ABclonal:Immunohistochemistry - PGC1α Rabbit pAb (A12348)

Immunohistochemistry of paraffin-embedded rat kidney using PGC1α Rabbit pAb (A12348) at dilution of 1:50 (40x lens).

ABclonal:Immunohistochemistry - PGC1α Rabbit pAb (A12348)

Immunohistochemistry of paraffin-embedded rat liver using PGC1α Rabbit pAb (A12348) at dilution of 1:50 (40x lens).

ABclonal:Immunofluorescence - PGC1α Rabbit pAb (A12348)

Immunofluorescence analysis of U2OS cells using PGC1α Rabbit pAb (A12348) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for PGC1α Rabbit pAb(A12348)
Review
ABclonal: review for PGC1α Rabbit pAb(A12348)

Overview

Product namePGC1α Rabbit pAb
Catalog No.A12348
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human PGC1α (NP_037393.1).
SequenceFGEIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDSNSDDFDPASTKSKYDSLDFDSLLKEAQRSLRR
Gene ID10891
Swiss protQ9UBK2
SynonymsPPARGC1A; LEM6; PGC-1(alpha); PGC-1alpha; PGC-1v; PGC1; PGC1A; PPARGC1; PPARG coactivator 1 alpha; PGC1 alpha
Calculated MW14kDa/30kDa/31kDa/33kDa/77kDa/89kDa/91kDa
Observed MW100KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, Mouse skeletal muscle, Mouse kidney, Rat kidney
Cellular locationCytoplasm, Nucleus, PML body
Customer validation

(mouse cells, Rabbit cerebral microvascular endothelial cells)

WB(Mus musculus, Homo sapiens, Gallus gallus, Rattus norvegicus)

IHC(Homo sapiens)

IF(Rattus norvegicus)

ABclonal:Western blot - PGC1α Rabbit pAb (A12348) }

Western blot - PGC1α Rabbit pAb (A12348)

Western blot analysis of extracts of various cell lines, using PGC1α antibody (A12348) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - PGC1α Rabbit pAb (A12348) }

Immunohistochemistry - PGC1α Rabbit pAb (A12348)

Immunohistochemistry of paraffin-embedded mouse heart using PGC1α Rabbit pAb (A12348) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - PGC1α Rabbit pAb (A12348) }

Immunohistochemistry - PGC1α Rabbit pAb (A12348)

Immunohistochemistry of paraffin-embedded rat kidney using PGC1α Rabbit pAb (A12348) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - PGC1α Rabbit pAb (A12348) }

Immunohistochemistry - PGC1α Rabbit pAb (A12348)

Immunohistochemistry of paraffin-embedded rat liver using PGC1α Rabbit pAb (A12348) at dilution of 1:50 (40x lens).
ABclonal:Immunofluorescence - PGC1α Rabbit pAb (A12348) }

Immunofluorescence - PGC1α Rabbit pAb (A12348)

Immunofluorescence analysis of U2OS cells using PGC1α Rabbit pAb (A12348) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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