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PGC1α Rabbit pAb (A12348)

Reviews (2)Publications (25) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - PGC1α Rabbit pAb (A12348)

Western blot analysis of extracts of various cell lines, using PGC1α antibody (A12348) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - PGC1α Rabbit pAb (A12348)

Western blot analysis of extracts of various cell lines, using PGC1α antibody (A12348) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - PGC1α Rabbit pAb (A12348)

Immunohistochemistry of paraffin-embedded human liver using PGC1α Rabbit pAb (A12348) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PGC1α Rabbit pAb (A12348)

Immunofluorescence analysis of HeLa cells using PGC1α Rabbit pAb (A12348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PGC1α Rabbit pAb (A12348)

Immunofluorescence analysis of HepG2 cells using PGC1α Rabbit pAb (A12348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PGC1α Rabbit pAb (A12348)

Immunofluorescence analysis of NIH/3T3 cells using PGC1α Rabbit pAb (A12348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PGC1α Rabbit pAb (A12348)

Immunofluorescence analysis of PC-12 cells using PGC1α Rabbit pAb (A12348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for PGC1α Rabbit pAb(A12348)
Review
ABclonal: review for PGC1α Rabbit pAb(A12348)

Overview

Product namePGC1α Rabbit pAb
Catalog No.A12348
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 610-798 of human PGC1α (NP_037393.1).
SequenceHRTHRNSPLYVRSRSRSPYSRRPRYDSYEEYQHERLKREEYRREYEKRESERAKQRERQRQKAIEERRVIYVGKIRPDTTRTELRDRFEVFGEIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDSNSDDFDPASTKSKYDSLDFDSLLKEAQRSLRR
Gene ID10891
Swiss protQ9UBK2
SynonymsPPARGC1A; LEM6; PGC-1(alpha); PGC-1alpha; PGC-1v; PGC1; PGC1A; PPARGC1; PPARG coactivator 1 alpha; PGC1 alpha
Calculated MW14kDa/30kDa/31kDa/33kDa/77kDa/89kDa/91kDa
Observed MW91KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HepG2, Mouse heart, Mouse skeletal muscle, Rat heart
Cellular locationCytoplasm, Nucleus, PML body
Customer validation

(mouse cells, Rabbit cerebral microvascular endothelial cells)

WB(Rattus norvegicus, Homo sapiens, Mus musculus, Gallus gallus, Hordeum vulgare)

IF(Rattus norvegicus)

IHC(Homo sapiens, Mus musculus)

ABclonal:Western blot - PGC1α Rabbit pAb (A12348)}

Western blot - PGC1α Rabbit pAb (A12348)

Western blot analysis of extracts of various cell lines, using PGC1α antibody (A12348) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - PGC1α Rabbit pAb (A12348)}

Western blot - PGC1α Rabbit pAb (A12348)

Western blot analysis of extracts of various cell lines, using PGC1α antibody (A12348) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - PGC1α Rabbit pAb (A12348)}

Immunohistochemistry - PGC1α Rabbit pAb (A12348)

Immunohistochemistry of paraffin-embedded human liver using PGC1α Rabbit pAb (A12348) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - PGC1α Rabbit pAb (A12348)}

Immunofluorescence - PGC1α Rabbit pAb (A12348)

Immunofluorescence analysis of HeLa cells using PGC1α Rabbit pAb (A12348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PGC1α Rabbit pAb (A12348)}

Immunofluorescence - PGC1α Rabbit pAb (A12348)

Immunofluorescence analysis of HepG2 cells using PGC1α Rabbit pAb (A12348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PGC1α Rabbit pAb (A12348)}

Immunofluorescence - PGC1α Rabbit pAb (A12348)

Immunofluorescence analysis of NIH/3T3 cells using PGC1α Rabbit pAb (A12348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PGC1α Rabbit pAb (A12348)}

Immunofluorescence - PGC1α Rabbit pAb (A12348)

Immunofluorescence analysis of PC-12 cells using PGC1α Rabbit pAb (A12348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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