Reviews (2)Publications (18) Datasheet
Western blot analysis of extracts of various cell lines, using PGC1α antibody (A12348) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry of paraffin-embedded mouse heart using PGC1α Rabbit pAb (A12348) at dilution of 1:50 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using PGC1α Rabbit pAb (A12348) at dilution of 1:50 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using PGC1α Rabbit pAb (A12348) at dilution of 1:50 (40x lens).
Immunofluorescence analysis of U2OS cells using PGC1α Rabbit pAb (A12348) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
| Product name | PGC1α Rabbit pAb |
|---|---|
| Catalog No. | A12348 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 700 to the C-terminus of human PGC1α (NP_037393.1). |
|---|---|
| Sequence | FGEIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDSNSDDFDPASTKSKYDSLDFDSLLKEAQRSLRR |
| Gene ID | 10891 |
| Swiss prot | Q9UBK2 |
| Synonyms | PPARGC1A; LEM6; PGC-1(alpha); PGC-1alpha; PGC-1v; PGC1; PGC1A; PPARGC1; PPARG coactivator 1 alpha; PGC1 alpha |
| Calculated MW | 14kDa/30kDa/31kDa/33kDa/77kDa/89kDa/91kDa |
| Observed MW | 100KDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | 293T, Mouse skeletal muscle, Mouse kidney, Rat kidney |
| Cellular location | Cytoplasm, Nucleus, PML body |
| Customer validation | (mouse cells, Rabbit cerebral microvascular endothelial cells) WB(Mus musculus, Homo sapiens, Gallus gallus, Rattus norvegicus) IHC(Homo sapiens) IF(Rattus norvegicus) |
* For research use only. Not for therapeutic or diagnostic purposes.
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