Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PFKP Rabbit pAb (A7916)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - PFKP Rabbit pAb (A7916)

Western blot analysis of various lysates using PFKP Rabbit pAb (A7916) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - PFKP Rabbit pAb (A7916)

Immunohistochemistry analysis of PFKP in paraffin-embedded rat brain using PFKP Rabbit pAb (A7916) (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PFKP Rabbit pAb (A7916)

Immunohistochemistry analysis of PFKP in paraffin-embedded mouse brain using PFKP Rabbit pAb (A7916) (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PFKP Rabbit pAb (A7916)

Immunofluorescence analysis of NIH/3T3 cells using PFKP Rabbit pAb (A7916) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PFKP Rabbit pAb (A7916)

Immunofluorescence analysis of U2OS cells using PFKP Rabbit pAb (A7916) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PFKP Rabbit pAb (A7916)

Immunofluorescence analysis of NIH/3T3 cells using PFKP Rabbit pAb (A7916) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name PFKP Rabbit pAb
Catalog No. A7916
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 336-540 of human PFKP (NP_002618.1).
Sequence ATPDTPACVVSLNGNHAVRLPLMECVQMTQDVQKAMDERRFQDAVRLRGRSFAGNLNTYKRLAIKLPDDQIPKTNCNVAVINVGAPAAGMNAAVRSAVRVGIADGHRMLAIYDGFDGFAKGQIKEIGWTDVGGWTGQGGSILGTKRVLPGKYLEEIATQMRTHSINALLIIGGFEAYLGLLELSAAREKHEEFCVPMVMVPATVS
Gene ID 5214
Swiss prot Q01813
Synonyms PFKF; PFK-C; PFK-P; ATP-PFK; PFKP
Calculated MW 86kDa
Observed MW 86kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:100 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples MCF7, Jurkat, Mouse brain, Mouse heart, Rat testis, Rat brain
Cellular location Cytoplasm

Documents

Certificate of Compliance

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Lot number

Research Area

PFKP Rabbit pAb images

ABclonal:Western blot - PFKP Rabbit pAb (A7916)}

Western blot - PFKP Rabbit pAb (A7916)

Western blot analysis of various lysates using PFKP Rabbit pAb (A7916) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - PFKP Rabbit pAb (A7916)}

Immunohistochemistry - PFKP Rabbit pAb (A7916)

Immunohistochemistry analysis of PFKP in paraffin-embedded rat brain using PFKP Rabbit pAb (A7916) (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PFKP Rabbit pAb (A7916)}

Immunohistochemistry - PFKP Rabbit pAb (A7916)

Immunohistochemistry analysis of PFKP in paraffin-embedded mouse brain using PFKP Rabbit pAb (A7916) (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - PFKP Rabbit pAb (A7916)}

Immunofluorescence - PFKP Rabbit pAb (A7916)

Immunofluorescence analysis of NIH/3T3 cells using PFKP Rabbit pAb (A7916) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PFKP Rabbit pAb (A7916)}

Immunofluorescence - PFKP Rabbit pAb (A7916)

Immunofluorescence analysis of U2OS cells using PFKP Rabbit pAb (A7916) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PFKP Rabbit pAb (A7916)}

Immunofluorescence - PFKP Rabbit pAb (A7916)

Immunofluorescence analysis of NIH/3T3 cells using PFKP Rabbit pAb (A7916) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on PFKP. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PFKP. (Distance between topics and target gene indicate popularity.) PFKP

* Data provided by citexs.com, for reference only.

Publishing research using A7916? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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