Products > Catalog Antibodies > Polyclonal Antibodies

CD31/PECAM1 Rabbit pAb (A11525)

Publications (5) Datasheet

ABclonal:Western blot - CD31/PECAM1 Rabbit pAb (A11525)


Product nameCD31/PECAM1 Rabbit pAb
Catalog No.A11525
Host speciesRabbit
Purification methodAffinity purification
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of mouse CD31/PECAM1 (NP_001292086.1).
SequenceEmail for sequence
Gene ID18613
Swiss protQ08481
SynonymsPECAM1; CD31; CD31/EndoCAM; GPIIA; PECA1; PECAM-1; endoCAM
Calculated MW79kDa/80kDa/81kDa/82kDa
Observed MW130kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesTHP-1, Mouse lung, Mouse spleen, Mouse heart
Cellular locationCell junction, Cell junction, Cell membrane, Lipid-anchor, Single-pass type I membrane protein
Customer validation

IF(Homo sapiens)

IHC(Homo sapiens, Mus musculus)

WB(Mus musculus)

    ABclonal:Western blot - CD31/PECAM1 Rabbit pAb (A11525) }

    Western blot - CD31/PECAM1 Rabbit pAb (A11525)

    Western blot analysis of extracts of various cell lines, using CD31/PECAM1 antibody (A11525) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    Inquire About This Product

    Submit your question about A11525 below and we will get back to you within one business day.

    Alternatively, call us at 888.754.5670.

    Publishing research using A11525? Please let us know so that we can cite the reference in this datasheet.

    Select size and quantity
    5 μL sample size available Add to Cart
    Contact us to order