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CD31/PECAM1 Rabbit pAb (A11525)

Publications (5) Datasheet

ABclonal:Western blot - CD31/PECAM1 Rabbit pAb (A11525)

Overview

Product nameCD31/PECAM1 Rabbit pAb
Catalog No.A11525
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of mouse CD31/PECAM1 (NP_001292086.1).
SequenceEmail for sequence
Gene ID18613
Swiss protQ08481
SynonymsPECAM1; CD31; CD31/EndoCAM; GPIIA; PECA1; PECAM-1; endoCAM
Calculated MW79kDa/80kDa/81kDa/82kDa
Observed MW130kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesTHP-1, Mouse lung, Mouse spleen, Mouse heart
Cellular locationCell junction, Cell junction, Cell membrane, Lipid-anchor, Single-pass type I membrane protein
Customer validation

IF(Homo sapiens)

IHC(Homo sapiens, Mus musculus)

WB(Mus musculus)

    ABclonal:Western blot - CD31/PECAM1 Rabbit pAb (A11525) }

    Western blot - CD31/PECAM1 Rabbit pAb (A11525)

    Western blot analysis of extracts of various cell lines, using CD31/PECAM1 antibody (A11525) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

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