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PEBP1 Rabbit mAb (A12768)

Datasheet

ABclonal:Western blot - PEBP1 Rabbit mAb (A12768)

Overview

Product namePEBP1 Rabbit mAb
Catalog No.A12768
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Jul 2015]
ImmunogenA synthesized peptide derived from human PEBP1
SequenceEmail for sequence
Gene ID5037
Swiss protP30086
SynonymsHCNP; HCNPpp; HEL-210; HEL-S-34; HEL-S-96; PBP; PEBP; PEBP-1; RKIP
Calculated MW21kDa
Observed MW21KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHepG2, Mouse liver, Mouse brain, Mouse thymus, Rat liver, Rat brain, Rat thymus
Cellular location
ABclonal:Western blot - PEBP1 Rabbit mAb (A12768) }

Western blot - PEBP1 Rabbit mAb (A12768)

Western blot analysis of extracts of various cell lines, using PEBP1 Rabbit mAb (A12768) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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