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PCNA Rabbit pAb (A0264)

Publications (21) Datasheet

ABclonal:Western blot - PCNA Rabbit pAb (A0264)

Western blot analysis of extracts of various cell lines, using PCNA antibody (A0264) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - PCNA Rabbit pAb (A0264)

Western blot analysis of extracts of various cell lines, using PCNA antibody (A0264) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - PCNA Rabbit pAb (A0264)

Immunohistochemistry of paraffin-embedded rat liver using PCNA antibody (A0264) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - PCNA Rabbit pAb (A0264)

Immunohistochemistry of paraffin-embedded human breast cancer using PCNA antibody (A0264) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - PCNA Rabbit pAb (A0264)

Immunohistochemistry of paraffin-embedded mouse testis using PCNA antibody (A0264) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - PCNA Rabbit pAb (A0264)

Immunofluorescence analysis of HeLa cells using PCNA antibody (A0264) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - PCNA Rabbit pAb (A0264)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug PCNA antibody (A0264). Western blot was performed from the immunoprecipitate using PCNA antibody (A0264) at a dilition of 1:1000.

Overview

Product namePCNA Rabbit pAb
Catalog No.A0264
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PCNA (NP_002583.1).
SequenceMFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS
Gene ID5111
Swiss protP12004
SynonymsPCNA; ATLD2
Calculated MW28kDa
Observed MW34KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, MCF7, 293T, C2C12, COS-7, Rat testis
Cellular locationNucleus
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Capra hircus)

IF(Rattus norvegicus, Homo sapiens)

IHC(Rattus norvegicus, Mus musculus)

IHC(Homo sapiens, Mus musculus)

ABclonal:Western blot - PCNA Rabbit pAb (A0264) }

Western blot - PCNA Rabbit pAb (A0264)

Western blot analysis of extracts of various cell lines, using PCNA antibody (A0264) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - PCNA Rabbit pAb (A0264) }

Western blot - PCNA Rabbit pAb (A0264)

Western blot analysis of extracts of various cell lines, using PCNA antibody (A0264) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - PCNA Rabbit pAb (A0264) }

Immunohistochemistry - PCNA Rabbit pAb (A0264)

Immunohistochemistry of paraffin-embedded rat liver using PCNA antibody (A0264) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PCNA Rabbit pAb (A0264) }

Immunohistochemistry - PCNA Rabbit pAb (A0264)

Immunohistochemistry of paraffin-embedded human breast cancer using PCNA antibody (A0264) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PCNA Rabbit pAb (A0264) }

Immunohistochemistry - PCNA Rabbit pAb (A0264)

Immunohistochemistry of paraffin-embedded mouse testis using PCNA antibody (A0264) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - PCNA Rabbit pAb (A0264) }

Immunofluorescence - PCNA Rabbit pAb (A0264)

Immunofluorescence analysis of HeLa cells using PCNA antibody (A0264) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - PCNA Rabbit pAb (A0264) }

Immunoprecipitation - PCNA Rabbit pAb (A0264)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug PCNA antibody (A0264). Western blot was performed from the immunoprecipitate using PCNA antibody (A0264) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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