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PCDHB16 Polyclonal Antibody (A15493)

Datasheet

ABclonal:Western blot - PCDHB16 Polyclonal Antibody (A15493)

Overview

Product namePCDHB16 Polyclonal Antibody
Catalog No.A15493
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 29-90 of human PCDHB16 (NP_066008.2).
SequenceELGSYSVVEETERGSFVANLGKDLGLGLTEMSTRKARIISQGNKQHLQLKAQTGDLLINEKL
Gene ID57717
Swiss protQ9NRJ7
SynonymsPCDHB16; ME1; PCDH-BETA16; PCDH3X; PCDHB8a; protocadherin beta-16
Calculated MW84kDa
Observed MW85kDa
ReactivityMouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMouse brain, Rat brain
Cellular locationMembrane, Single-pass type I membrane protein
ABclonal:Western blot - PCDHB16 Polyclonal Antibody (A15493) }

Western blot - PCDHB16 Polyclonal Antibody (A15493)

Western blot analysis of extracts of various cell lines, using PCDHB16 antibody (A15493) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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