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PARP1 Rabbit pAb (A0010)

Publication (1) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - PARP1 Rabbit pAb (A0010)

Western blot analysis of extracts of various cell lines, using PARP1 antibody (A0010) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - PARP1 Rabbit pAb (A0010)

Immunohistochemistry of paraffin-embedded rat testis using PARP1 antibody (A0010) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PARP1 Rabbit pAb (A0010)

Immunohistochemistry of paraffin-embedded human tonsil using PARP1 antibody (A0010) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - PARP1 Rabbit pAb (A0010)

Immunohistochemistry of paraffin-embedded mouse kidney using PARP1 antibody (A0010) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - PARP1 Rabbit pAb (A0010)

Immunofluorescence analysis of THP-1 using PARP1 antibody (A0010) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PARP1 Rabbit pAb (A0010)

Immunofluorescence analysis of L929 using PARP1 antibody (A0010) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PARP1 Rabbit pAb (A0010)

Immunofluorescence analysis of C6 using PARP1 antibody (A0010) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product namePARP1 Rabbit pAb
Catalog No.A0010
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 662-1014 of PARP1 (NP_001609.2).
SequenceKSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW
Gene ID142
Swiss protP09874
SynonymsADPRT; ADPRT 1; ADPRT1; ARTD1; PARP; PARP-1; PPOL; pADPRT-1; PARP1; Cleaved PARP; PARP1
Calculated MW113kDa
Observed MW116KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC 1:50 - 1:200
  • IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293F, Mouse placenta
Cellular locationNucleus, nucleolus
Customer validation

WB(Homo sapiens,Mus musculus)

ABclonal:Western blot - PARP1 Rabbit pAb (A0010)}

Western blot - PARP1 Rabbit pAb (A0010)

Western blot analysis of extracts of various cell lines, using PARP1 antibody (A0010) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - PARP1 Rabbit pAb (A0010)}

Immunohistochemistry - PARP1 Rabbit pAb (A0010)

Immunohistochemistry of paraffin-embedded rat testis using PARP1 antibody (A0010) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PARP1 Rabbit pAb (A0010)}

Immunohistochemistry - PARP1 Rabbit pAb (A0010)

Immunohistochemistry of paraffin-embedded human tonsil using PARP1 antibody (A0010) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - PARP1 Rabbit pAb (A0010)}

Immunohistochemistry - PARP1 Rabbit pAb (A0010)

Immunohistochemistry of paraffin-embedded mouse kidney using PARP1 antibody (A0010) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - PARP1 Rabbit pAb (A0010)}

Immunofluorescence - PARP1 Rabbit pAb (A0010)

Immunofluorescence analysis of THP-1 using PARP1 antibody (A0010) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PARP1 Rabbit pAb (A0010)}

Immunofluorescence - PARP1 Rabbit pAb (A0010)

Immunofluorescence analysis of L929 using PARP1 antibody (A0010) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PARP1 Rabbit pAb (A0010)}

Immunofluorescence - PARP1 Rabbit pAb (A0010)

Immunofluorescence analysis of C6 using PARP1 antibody (A0010) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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