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PARP1 Polyclonal Antibody (A0942)

Review (1) Datasheet

ABclonal:Western blot - PARP1 Polyclonal Antibody (A0942)
ABclonal:Immunohistochemistry - PARP1 Polyclonal Antibody (A0942)
ABclonal:Immunohistochemistry - PARP1 Polyclonal Antibody (A0942)
ABclonal:Immunohistochemistry - PARP1 Polyclonal Antibody (A0942)
ABclonal:Immunohistochemistry - PARP1 Polyclonal Antibody (A0942)
ABclonal:Immunohistochemistry - PARP1 Polyclonal Antibody (A0942)
ABclonal:Immunohistochemistry - PARP1 Polyclonal Antibody (A0942)
ABclonal:Immunofluorescence - PARP1 Polyclonal Antibody (A0942)

Overview

Product namePARP1 Polyclonal Antibody
Catalog No.A0942
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 81-390 of human PARP1 (NP_001609.2).
SequenceDQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPL
Gene ID142
Swiss protP09874
SynonymsADPRT; ADPRT1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PPOL
Calculated MW113kDa
Observed MW115kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:1000 - 1:3000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-87MG, SKOV3, HeLa, Jurkat, HepG2
Cellular locationNucleus, nucleolus
Customer validation

WB(HCC827)

ABclonal:Western blot - PARP1 Polyclonal Antibody (A0942) }

Western blot - PARP1 Polyclonal Antibody (A0942)

Western blot analysis of extracts of various cell lines, using PARP1 antibody (A0942) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - PARP1 Polyclonal Antibody (A0942) }

Immunohistochemistry - PARP1 Polyclonal Antibody (A0942)

Immunohistochemistry of paraffin-embedded rat testis using PARP1 Antibody (A0942) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PARP1 Polyclonal Antibody (A0942) }

Immunohistochemistry - PARP1 Polyclonal Antibody (A0942)

Immunohistochemistry of paraffin-embedded human vermiform appendix using PARP1 Antibody (A0942) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PARP1 Polyclonal Antibody (A0942) }

Immunohistochemistry - PARP1 Polyclonal Antibody (A0942)

Immunohistochemistry of paraffin-embedded human breast cancer using PARP1 Antibody (A0942) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PARP1 Polyclonal Antibody (A0942) }

Immunohistochemistry - PARP1 Polyclonal Antibody (A0942)

Immunohistochemistry of paraffin-embedded human tonsil using PARP1 antibody (A0942) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PARP1 Polyclonal Antibody (A0942) }

Immunohistochemistry - PARP1 Polyclonal Antibody (A0942)

Immunohistochemistry of paraffin-embedded human appendix using PARP1 antibody (A0942) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - PARP1 Polyclonal Antibody (A0942) }

Immunohistochemistry - PARP1 Polyclonal Antibody (A0942)

Immunohistochemistry of paraffin-embedded human mammary cancer using PARP1 antibody (A0942) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - PARP1 Polyclonal Antibody (A0942) }

Immunofluorescence - PARP1 Polyclonal Antibody (A0942)

Immunofluorescence analysis of U2OS cells using PARP1 antibody (A0942) at dilution of 1:100. Blue: DAPI for nuclear staining.

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