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PABPN1 Polyclonal Antibody (A6041)

Publications (2) Datasheet

ABclonal:Western blot - PABPN1 Polyclonal Antibody (A6041)

Overview

Product namePABPN1 Polyclonal Antibody
Catalog No.A6041
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. This gene contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human PABPN1 (NP_004634.1).
SequenceMAAAAAAAAAAGAAGGRGSGPGRRRHLVPGAGGEAGEGAPGGAGDYGNGLESEELEPEELLLEPEPEPEPEEEPPRPRAPPGAPGPGPGSGAPGSQEEEE
Gene ID8106
Swiss protQ86U42
SynonymsPABPN1; OPMD; PAB2; PABII; PABP-2; PABP2; polyadenylate-binding protein 2
Calculated MW31kDa/32kDa/37kDa
Observed MW45kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesB cells, HeLa, HepG2, U-87MG, Mouse brain, Mouse testis
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Human)

IF(Human)

ABclonal:Western blot - PABPN1 Polyclonal Antibody (A6041) }

Western blot - PABPN1 Polyclonal Antibody (A6041)

Western blot analysis of extracts of various cell lines, using PABPN1 antibody (A6041) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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