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P90RSK Polyclonal Antibody (A15718)

Datasheet

ABclonal:Western blot - P90RSK Polyclonal Antibody (A15718)
ABclonal:Immunohistochemistry - P90RSK Polyclonal Antibody (A15718)
ABclonal:Immunofluorescence - P90RSK Polyclonal Antibody (A15718)
ABclonal:Immunofluorescence - P90RSK Polyclonal Antibody (A15718)

Overview

Product nameP90RSK Polyclonal Antibody
Catalog No.A15718
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of human P90RSK (NP_002944.2).
SequenceLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL
Gene ID6195
Swiss protQ15418
SynonymsRPS6KA1; HU-1; MAPKAPK1A; RSK; RSK1; p90Rsk; ribosomal protein S6 kinase A1
Calculated MW72kDa/81kDa/82kDa/83kDa
Observed MW90kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHT-29, HeLa, MCF7, K-562, Mouse lung, Mouse brain
Cellular locationCytoplasm, Nucleus
ABclonal:Western blot - P90RSK Polyclonal Antibody (A15718) }

Western blot - P90RSK Polyclonal Antibody (A15718)

Western blot analysis of extracts of various cell lines, using P90RSK antibody (A15718) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - P90RSK Polyclonal Antibody (A15718) }

Immunohistochemistry - P90RSK Polyclonal Antibody (A15718)

Immunohistochemistry of paraffin-embedded mouse spleen using RPS6KA1 antibody (A15718) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - P90RSK Polyclonal Antibody (A15718) }

Immunofluorescence - P90RSK Polyclonal Antibody (A15718)

Immunofluorescence analysis of L929 cells using RPS6KA1 antibody (A15718) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - P90RSK Polyclonal Antibody (A15718) }

Immunofluorescence - P90RSK Polyclonal Antibody (A15718)

Immunofluorescence analysis of U-2 OS cells using RPS6KA1 antibody (A15718) at dilution of 1:100. Blue: DAPI for nuclear staining.

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