Products > Catalog Antibodies > Polyclonal Antibodies

Osteopontin Rabbit pAb (A1361)

Publications (7) Datasheet

ABclonal:Western blot - Osteopontin Rabbit pAb (A1361)

Western blot analysis of extracts of various cell lines, using Osteopontin antibody (A1361) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameOsteopontin Rabbit pAb
Catalog No.A1361
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 17-314 of human Osteopontin (NP_001035147.1).
SequenceIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN
Gene ID6696
Swiss protP10451
SynonymsBNSP; BSPI; ETA-1; OPN; Osteopontin; SPP1
Calculated MW32kDa/33kDa/35kDa
Observed MW65KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMouse liver, Mouse kidney, Rat kidney
Cellular locationSecreted
Customer validation

IHC(Oryza sativa)

WB(Homo sapiens)

ABclonal:Western blot - Osteopontin Rabbit pAb (A1361) }

Western blot - Osteopontin Rabbit pAb (A1361)

Western blot analysis of extracts of various cell lines, using Osteopontin antibody (A1361) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

Inquire About This Product

Submit your question about A1361 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A1361? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μL sample size available Add to Cart
Contact us to order