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Occludin Rabbit pAb (A2601)

Reviews (2)Publications (18) Datasheet

ABclonal:Occludin Rabbit pAb
ABclonal:Western blot - Occludin Rabbit pAb (A2601)

Western blot analysis of extracts of Mouse liver, using Occludin antibody (A2601) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Occludin Rabbit pAb (A2601)

Western blot analysis of extracts of various cell lines, using Occludin antibody (A2601) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - Occludin Rabbit pAb (A2601)

Western blot analysis of extracts of Rat kidney, using Occludin antibody (A2601) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry of paraffin-embedded rat ovary using Occludin Rabbit pAb (A2601) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry of paraffin-embedded human colon using Occludin Rabbit pAb (A2601) at dilution of 1:100 (40x lens).

Review
ABclonal: review for Occludin Rabbit pAb(A2601)
Review
ABclonal: review for Occludin Rabbit pAb(A2601)

Overview

Product nameOccludin Rabbit pAb
Catalog No.A2601
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 263-522 of human Occludin (NP_002529.1).
SequenceFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT
Gene ID100506658
Swiss protQ16625
SynonymsOCLN; BLCPMG; PPP1R115; PTORCH1; occludin; Occludin
Calculated MW8kDa/23kDa/31kDa/52kDa/54kDa/59kDa
Observed MW60KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:1000 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa, 293T, Mouse liver, Rat kidney
Cellular locationCell junction, Membrane, Multi-pass membrane protein, tight junction
Customer validation

(Mouse embryonal fibroblast cell, Pig cells)

WB(Rattus norvegicus, Mus musculus, Homo sapiens, Sus scrofa)

IF(Mus musculus)

IHC(Rattus norvegicus)

IHC(Rattus norvegicus)

WB(Rattus norvegicus)

ABclonal:Western blot - Occludin Rabbit pAb (A2601) }

Western blot - Occludin Rabbit pAb (A2601)

Western blot analysis of extracts of Mouse liver, using Occludin antibody (A2601) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Occludin Rabbit pAb (A2601) }

Western blot - Occludin Rabbit pAb (A2601)

Western blot analysis of extracts of various cell lines, using Occludin antibody (A2601) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - Occludin Rabbit pAb (A2601) }

Western blot - Occludin Rabbit pAb (A2601)

Western blot analysis of extracts of Rat kidney, using Occludin antibody (A2601) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601) }

Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry of paraffin-embedded rat ovary using Occludin Rabbit pAb (A2601) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Occludin Rabbit pAb (A2601) }

Immunohistochemistry - Occludin Rabbit pAb (A2601)

Immunohistochemistry of paraffin-embedded human colon using Occludin Rabbit pAb (A2601) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

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