Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

OS9 Rabbit pAb (A12261)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - OS9 Rabbit pAb (A12261)

Western blot analysis of various lysates using OS9 Rabbit pAb (A12261) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

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Overview

Product name OS9 Rabbit pAb
Catalog No. A12261
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a protein that is highly expressed in osteosarcomas. This protein binds to the hypoxia-inducible factor 1 (HIF-1), a key regulator of the hypoxic response and angiogenesis, and promotes the degradation of one of its subunits. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 353-612 of human OS9 (NP_001017956.1).
Sequence NVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDLDEFDF
Gene ID 10956
Swiss prot Q13438
Synonyms OS-9; ERLEC2; OS9
Calculated MW 76kDa
Observed MW 70kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples U-87MG, LO2, BxPC-3, B cells, HeLa, Mouse liver
Cellular location Endoplasmic reticulum lumen

Research Area

OS9 Rabbit pAb images

ABclonal:Western blot - OS9 Rabbit pAb (A12261)}

Western blot - OS9 Rabbit pAb (A12261)

Western blot analysis of various lysates using OS9 Rabbit pAb (A12261) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

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Related Targets

Based on existing publications, the following genes are closely related to research on OS9. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to OS9. (Distance between topics and target gene indicate popularity.) OS9

* Data provided by citexs.com, for reference only.

Publishing research using A12261? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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