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OPA1 Rabbit pAb (A9833)

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ABclonal:Western blot - OPA1 Rabbit pAb (A9833)

Western blot analysis of extracts of various cell lines, using OPA1 Rabbit pAb (A9833) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Review
ABclonal: review for OPA1 Rabbit pAb(A9833)
Review
ABclonal: review for OPA1 Rabbit pAb(A9833)

Overview

Product nameOPA1 Rabbit pAb
Catalog No.A9833
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene product is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 661-960 of human OPA1 (NP_056375.2).
SequenceTDKQLPNKAVEVAWETLQEEFSRFMTEPKGKEHDDIFDKLKEAVKEESIKRHKWNDFAEDSLRVIQHNALEDRSISDKQQWDAAIYFMEEALQARLKDTENAIENMVGPDWKKRWLYWKNRTQEQCVHNETKNELEKMLKCNEEHPAYLASDEITTVRKNLESRGVEVDPSLIKDTWHQVYRRHFLKTALNHCNLCRRGFYYYQRHFVDSELECNDVVLFWRIQRMLAITANTLRQQLTNTEVRRLEKNVKEVLEDFAEDGEKKIKLLTGKRVQLAEDLKKVREIQEKLDAFIEALHQEK
Gene ID4976
Swiss protO60313
SynonymsOPA1; BERHS; MGM1; MTDPS14; NPG; NTG; largeG
Calculated MW111kDa/115kDa
Observed MW80-100KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, HepG2, U-251MG, Mouse liver, Mouse brain, Mouse heart, Rat liver, Rat brain
Cellular locationMitochondrion inner membrane, Mitochondrion intermembrane space, Single-pass membrane protein
Customer validation

(Human bone marrow cancer cell)

WB(Gallus gallus, Rattus norvegicus, Homo sapiens, Mus musculus)

IF(Rattus norvegicus)

ABclonal:Western blot - OPA1 Rabbit pAb (A9833) }

Western blot - OPA1 Rabbit pAb (A9833)

Western blot analysis of extracts of various cell lines, using OPA1 Rabbit pAb (A9833) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

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