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OGG1 Polyclonal Antibody (A2268)

Publication (1) Datasheet

ABclonal:Western blot - OGG1 Polyclonal Antibody (A2268)

Overview

Product nameOGG1 Polyclonal Antibody
Catalog No.A2268
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined.
ImmunogenA synthetic peptide of human OGG1
SequenceEmail for sequence
Gene ID4968
Swiss protO15527
SynonymsOGG1; HMMH; HOGG1; MUTM; OGH1; N-glycosylase/DNA lyase
Calculated MW22kDa/36kDa/38kDa/39kDa/40kDa/45kDa/47kDa
Observed MW36kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:200 - 1:1000
Storage bufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application keyWestern blotting    
Positive samplesHepG2
Cellular locationMitochondrion, Nucleus, Nucleus matrix, Nucleus speckle, nucleoplasm
Customer validation

WB(Human)

ABclonal:Western blot - OGG1 Polyclonal Antibody (A2268) }

Western blot - OGG1 Polyclonal Antibody (A2268)

Western blot analysis of extracts of HepG2 cells, using OGG1 antibody (A2268) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

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