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OGDH Polyclonal Antibody (A6391)

Publication (1) Datasheet

ABclonal:Western blot - OGDH Polyclonal Antibody (A6391)
ABclonal:Immunohistochemistry - OGDH Polyclonal Antibody (A6391)
ABclonal:Immunohistochemistry - OGDH Polyclonal Antibody (A6391)
ABclonal:Immunofluorescence - OGDH Polyclonal Antibody (A6391)

Overview

Product nameOGDH Polyclonal Antibody
Catalog No.A6391
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 148-427 of human OGDH (NP_001003941.1).
SequenceDPLGILDADLDSSVPADIISSTDKLGFYGLDESDLDKVFHLPTTTFIGGQESALPLREIIRRLEMAYCQHIGVEFMFINDLEQCQWIRQKFETPGIMQFTNEEKRTLLARLVRSTRFEEFLQRKWSSEKRFGLEGCEVLIPALKTIIDKSSENGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFDSKLEAADEGSGDVKYHLGMYHRRINRVTDRNITLSLVANPSHLEAADPVVMGKTKAEQFYCGDTEGKKVRPRERRARQIVKAPCSSMEFRSPT
Gene ID4967
Swiss protQ02218
SynonymsOGDH; AKGDH; E1k; OGDC; oxoglutarate dehydrogenase
Calculated MW48kDa/115kDa
Observed MW116kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse kidney, Mouse heart, Mouse brain, Rat kidney
Cellular locationMitochondrion matrix
Customer validation

WB(Human)

ABclonal:Western blot - OGDH Polyclonal Antibody (A6391) }

Western blot - OGDH Polyclonal Antibody (A6391)

Western blot analysis of extracts of various cell lines, using OGDH antibody (A6391) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
ABclonal:Immunohistochemistry - OGDH Polyclonal Antibody (A6391) }

Immunohistochemistry - OGDH Polyclonal Antibody (A6391)

Immunohistochemistry of paraffin-embedded rat ovary using OGDH antibody (A6391) at dilution of 1:100 (20x lens).
ABclonal:Immunohistochemistry - OGDH Polyclonal Antibody (A6391) }

Immunohistochemistry - OGDH Polyclonal Antibody (A6391)

Immunohistochemistry of paraffin-embedded human lung cancer using OGDH antibody (A6391) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - OGDH Polyclonal Antibody (A6391) }

Immunofluorescence - OGDH Polyclonal Antibody (A6391)

Immunofluorescence analysis of U2OS cells using OGDH antibody (A6391). Blue: DAPI for nuclear staining.

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