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OCT4 Polyclonal Antibody (A7920)

Review (2)Publication (5) Datasheet

ABclonal:Western blot - OCT4 Polyclonal Antibody (A7920)
ABclonal:Immunohistochemistry - OCT4 Polyclonal Antibody (A7920)
Review
ABclonal: review for OCT4 Polyclonal Antibody(A7920)
Review
ABclonal: review for OCT4 Polyclonal Antibody(A7920)

Overview

Product nameOCT4 Polyclonal Antibody
Catalog No.A7920
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
ImmunogenA synthetic peptide of human OCT4
SequenceEmail for sequence
Gene ID5460
Swiss protQ01860
SynonymsPOU5F1; OCT3; OCT4; OTF-3; OTF3; OTF4; Oct-3; Oct-4
Calculated MW30kDa/38kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesNIH/3T3, Mouse testis, Mouse brain, Mouse ovary, Rat testis
Cellular locationCytoplasm, Nucleus
Customer validation

(, )

IHC(Human, Mouse)

WB(Human)

IHC(Human)

ABclonal:Western blot - OCT4 Polyclonal Antibody (A7920) }

Western blot - OCT4 Polyclonal Antibody (A7920)

Western blot analysis of extracts of various cell lines, using OCT4 antibody (A7920) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - OCT4 Polyclonal Antibody (A7920) }

Immunohistochemistry - OCT4 Polyclonal Antibody (A7920)

Immunohistochemistry of paraffin-embedded human brain cancer using OCT4 antibody (A7920) at dilution of 1:100 (40x lens).

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