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OAT Polyclonal Antibody (A6235)

Publication (1) Datasheet

ABclonal:Western blot - OAT Polyclonal Antibody (A6235)
ABclonal:Western blot - OAT Polyclonal Antibody (A6235)
ABclonal:Immunoprecipitation - OAT Polyclonal Antibody (A6235)

Overview

Product nameOAT Polyclonal Antibody
Catalog No.A6235
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 160-439 of human OAT (NP_000265.1).
SequenceVKGIQKYKAKIVFAAGNFWGRTLSAISSSTDPTSYDGFGPFMPGFDIIPYNDLPALERALQDPNVAAFMVEPIQGEAGVVVPDPGYLMGVRELCTRHQVLFIADEIQTGLARTGRWLAVDYENVRPDIVLLGKALSGGLYPVSAVLCDDDIMLTIKPGEHGSTYGGNPLGCRVAIAALEVLEEENLAENADKLGIILRNELMKLPSDVVTAVRGKGLLNAIVIKETKDWDAWKVCLRLRDNGLLAKPTHGDIIRFAPPLVIKEDELRESIEIINKTILSF
Gene ID4942
Swiss protP04181
SynonymsOAT; GACR; HOGA; OATASE; OKT; ornithine aminotransferase
Calculated MW32kDa/48kDa
Observed MW47kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesSW480, MCF7, 293T, A-549, HepG2, Mouse liver, Mouse lung, Mouse kidney, Rat kidney
Cellular locationMitochondrion matrix
Customer validation

WB(Rat)

ABclonal:Western blot - OAT Polyclonal Antibody (A6235) }

Western blot - OAT Polyclonal Antibody (A6235)

Western blot analysis of extracts of various cell lines, using OAT antibody (A6235) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - OAT Polyclonal Antibody (A6235) }

Western blot - OAT Polyclonal Antibody (A6235)

Western blot analysis of extracts of various cells, using OAT antibody (A6235) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunoprecipitation - OAT Polyclonal Antibody (A6235) }

Immunoprecipitation - OAT Polyclonal Antibody (A6235)

Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug OAT antibody (A6235). Western blot was performed from the immunoprecipitate using OAT antibody (A6235) at a dilition of 1:1000.

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