Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Neurofilament L Rabbit pAb (A0257)

Review (1) Publications (4) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Neurofilament L Rabbit pAb (A0257)

Western blot analysis of various lysates using Neurofilament L Rabbit pAb (A0257) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Neurofilament L Rabbit pAb (A0257)

Immunohistochemistry analysis of Neurofilament L in paraffin-embedded human breast cancer using Neurofilament L Rabbit pAb (A0257) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Neurofilament L Rabbit pAb (A0257)

Immunohistochemistry analysis of Neurofilament L in paraffin-embedded rat kidney using Neurofilament L Rabbit pAb (A0257) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Neurofilament L Rabbit pAb (A0257)

Immunohistochemistry analysis of Neurofilament L in paraffin-embedded rat brain using Neurofilament L Rabbit pAb (A0257) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Neurofilament L Rabbit pAb (A0257)

Immunofluorescence analysis of paraffin-embedded rat brain using Neurofilament L Rabbit pAb (A0257) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Neurofilament L Rabbit pAb (A0257)

Immunofluorescence analysis of paraffin-embedded mouse brain using Neurofilament L Rabbit pAb (A0257) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Neurofilament L Rabbit pAb(A0257)

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Overview

Product name Neurofilament L Rabbit pAb
Catalog No. A0257
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human Neurofilament L (NP_006149.2).
Sequence SSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVT
Gene ID 4747
Swiss prot P07196
Synonyms NFL; NF-L; NF68; CMT1F; CMT2E; CMTDIG; PPP1R110; Neurofilament L
Calculated MW 62kDa
Observed MW 70kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples 293T, SH-SY5Y, U-251MG, Mouse brain, Rat brain
Cellular location axon, axon cytoplasm, cholinergic synapse, cytoplasm, cytosol, growth cone, neurofilament, neuromuscular junction, postsynaptic intermediate filament cytoskeleton, presynaptic intermediate filament cytoskeleton, Schaffer collateral - CA1 synapse
Customer validation

IF (Mouse tibialis anterior muscleMacaca mulatta, Homo sapiens, Macaca mulatta, Gallus gallus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Neurofilament L Rabbit pAb images

ABclonal:Western blot - Neurofilament L Rabbit pAb (A0257)}

Western blot - Neurofilament L Rabbit pAb (A0257)

Western blot analysis of various lysates using Neurofilament L Rabbit pAb (A0257) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Neurofilament L Rabbit pAb (A0257)}

Immunohistochemistry - Neurofilament L Rabbit pAb (A0257)

Immunohistochemistry analysis of Neurofilament L in paraffin-embedded human breast cancer using Neurofilament L Rabbit pAb (A0257) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Neurofilament L Rabbit pAb (A0257)}

Immunohistochemistry - Neurofilament L Rabbit pAb (A0257)

Immunohistochemistry analysis of Neurofilament L in paraffin-embedded rat kidney using Neurofilament L Rabbit pAb (A0257) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Neurofilament L Rabbit pAb (A0257)}

Immunohistochemistry - Neurofilament L Rabbit pAb (A0257)

Immunohistochemistry analysis of Neurofilament L in paraffin-embedded rat brain using Neurofilament L Rabbit pAb (A0257) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Neurofilament L Rabbit pAb (A0257)}

Immunofluorescence - Neurofilament L Rabbit pAb (A0257)

Immunofluorescence analysis of paraffin-embedded rat brain using Neurofilament L Rabbit pAb (A0257) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Neurofilament L Rabbit pAb (A0257)}

Immunofluorescence - Neurofilament L Rabbit pAb (A0257)

Immunofluorescence analysis of paraffin-embedded mouse brain using Neurofilament L Rabbit pAb (A0257) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on NEFL. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to NEFL. (Distance between topics and target gene indicate popularity.) NEFL

* Data provided by citexs.com, for reference only.

Publishing research using A0257? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

ELISA Kits (1)

Proteins (2)

Secondary Antibodies (23)

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