Products > Catalog Antibodies > Monoclonal Antibodies

Neurofilament H Rabbit mAb (A19084)

Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - Neurofilament H Rabbit mAb (A19084)

Western blot analysis of extracts of various cell lines, using Neurofilament H antibody (A19084) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Neurofilament H Rabbit mAb (A19084)

Immunohistochemistry of paraffin-embedded mouse brain using Neurofilament H Rabbit mAb (A19084) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Neurofilament H Rabbit mAb (A19084)

Immunohistochemistry of paraffin-embedded rat brain using Neurofilament H Rabbit mAb (A19084) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Neurofilament H Rabbit mAb (A19084)

Immunofluorescence analysis of rat brain using Neurofilament H Rabbit mAb (A19084) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Neurofilament H Rabbit mAb (A19084)

Immunoprecipitation analysis of 600ug extracts of Rat brain cells using 3ug Neurofilament H antibody (A19084). Western blot was performed from the immunoprecipitate using Neurofilament H antibody (A19084) at a dilition of 1:500.

ABclonal:Immunoprecipitation - Neurofilament H Rabbit mAb (A19084)

Immunoprecipitation analysis of 300ug extracts of PC-3 cells using 3ug Neurofilament H antibody (A19084). Western blot was performed from the immunoprecipitate using Neurofilament H antibody (A19084) at a dilition of 1:500.

Overview

Product nameNeurofilament H Rabbit mAb
Catalog No.A19084
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 550-650 of human Neurofilament H (P12036).
SequenceAKSPAKEEAKSPPEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKSPAEAKSPEKAKSPVKEEAKSPAEAKSPVKEEAKSPAEVKSPEKAKSPTKEEAKSPE
Gene ID4744
Swiss protP12036
SynonymsCMT2CC; NFH; NEFH
Calculated MW200kDa
Observed MW65kDa/220kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF 1:50 - 1:200
  • IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesPC-3, Mouse brain, Rat brain
Cellular location
ABclonal:Western blot - Neurofilament H Rabbit mAb (A19084)}

Western blot - Neurofilament H Rabbit mAb (A19084)

Western blot analysis of extracts of various cell lines, using Neurofilament H antibody (A19084) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Neurofilament H Rabbit mAb (A19084)}

Immunohistochemistry - Neurofilament H Rabbit mAb (A19084)

Immunohistochemistry of paraffin-embedded mouse brain using Neurofilament H Rabbit mAb (A19084) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Neurofilament H Rabbit mAb (A19084)}

Immunohistochemistry - Neurofilament H Rabbit mAb (A19084)

Immunohistochemistry of paraffin-embedded rat brain using Neurofilament H Rabbit mAb (A19084) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Neurofilament H Rabbit mAb (A19084)}

Immunofluorescence - Neurofilament H Rabbit mAb (A19084)

Immunofluorescence analysis of rat brain using Neurofilament H Rabbit mAb (A19084) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Neurofilament H Rabbit mAb (A19084)}

Immunoprecipitation - Neurofilament H Rabbit mAb (A19084)

Immunoprecipitation analysis of 600ug extracts of Rat brain cells using 3ug Neurofilament H antibody (A19084). Western blot was performed from the immunoprecipitate using Neurofilament H antibody (A19084) at a dilition of 1:500.
ABclonal:Immunoprecipitation - Neurofilament H Rabbit mAb (A19084)}

Immunoprecipitation - Neurofilament H Rabbit mAb (A19084)

Immunoprecipitation analysis of 300ug extracts of PC-3 cells using 3ug Neurofilament H antibody (A19084). Western blot was performed from the immunoprecipitate using Neurofilament H antibody (A19084) at a dilition of 1:500.

Inquire About This Product

Submit your question about A19084 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order