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Nestin Rabbit pAb (A11861)

Review (1)Publication (1) Datasheet

ABclonal:Western blot - Nestin Polyclonal Antibody (A11861)
ABclonal:Immunohistochemistry - Nestin Polyclonal Antibody (A11861)
ABclonal:Immunofluorescence - Nestin Polyclonal Antibody (A11861)
Review
ABclonal: review for Nestin Rabbit pAb(A11861)

Overview

Product nameNestin Rabbit pAb
Catalog No.A11861
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1392-1621 of human Nestin (NP_006608.1).
SequenceQVPQLLLDPAAWDRDGESDGFADEEESGEEGEEDQEEGREPGAGRWGPGSSVGSLQALSSSQRGEFLESDSVSVSVPWDDSLRGAVAGAPKTALETESQDSAEPSGSEEESDPVSLEREDKVPGPLEIPSGMEDAGPGADIIGVNGQGPNLEGKSQHVNGGVMNGLEQSEEVGQGMPLVSEGDRGSPFQEEEGSALKTSWAGAPVHLGQGQFLKFTQREGDRESWSSGED
Gene ID10763
Swiss protP48681
SynonymsNES; Nbla00170; nestin; Nestin
Calculated MW177kDa
Observed MW180kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesLO2, 293T, Mouse brain
Cellular location
Customer validation

(Mouse Monocyte Macrophage Leukemia Cells)

WB(Homo sapiens)

ABclonal:Western blot - Nestin Polyclonal Antibody (A11861) }

Western blot - Nestin Polyclonal Antibody (A11861)

Western blot analysis of extracts of various cell lines, using Nestin antibody (A11861) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Nestin Polyclonal Antibody (A11861) }

Immunohistochemistry - Nestin Polyclonal Antibody (A11861)

Immunohistochemistry of paraffin-embedded mouse brain using Nestin antibody (A11861) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Nestin Polyclonal Antibody (A11861) }

Immunofluorescence - Nestin Polyclonal Antibody (A11861)

Immunofluorescence analysis of U-251MG cells using Nestin antibody (A11861) at dilution of 1:100 (20x lens). Blue: DAPI for nuclear staining.

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