Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

TrkB Mouse mAb (A0487)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - TrkB Mouse mAb (A0487)

Western blot analysis of lysates from U-251MG cells, using TrkB Mouse mAb (A0487) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

You may also interested in:

Overview

Product name TrkB Mouse mAb
Catalog No. A0487
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen A synthetic peptide of human TrkB
Sequence Email for sequence
Gene ID 4915
Swiss prot Q16620
Synonyms OBHD; TRKB; DEE58; trk-B; EIEE58; GP145-TrkB; TrkB
Calculated MW 92kDa
Observed MW 140kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:20 - 1:50
  • FC 1:20 - 1:50
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    Immunohistochemistry    Flow Cytometry    
Positive samples U-251MG
Cellular location Cell membrane, Endosome membrane, Single-pass type I membrane protein
Customer validation

WB (Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

TrkB Mouse mAb images

ABclonal:Western blot - TrkB Mouse mAb (A0487)}

Western blot - TrkB Mouse mAb (A0487)

Western blot analysis of lysates from U-251MG cells, using TrkB Mouse mAb (A0487) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Inquire About This Product

Submit your question about A0487 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on NTRK2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to NTRK2. (Distance between topics and target gene indicate popularity.) NTRK2

* Data provided by citexs.com, for reference only.

Publishing research using A0487? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

!OUT OF STOCK

See Below for Alternatives
Contact us to order