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NTRK2 Monoclonal Antibody (A0487)

Publication (1) Datasheet

ABclonal:Western blot - NTRK2 antibody (A0487)

Overview

Product nameNTRK2 Monoclonal Antibody
Catalog No.A0487
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide of human NTRK2
SequenceEmail for sequence
Gene ID4915
Swiss protQ16620
SynonymsGP145-TrkB; OBHD; trk-B; TRKB; GP145-TrkB; trk-B; TRKB
Calculated MW35kDa/53kDa/59kDa/60kDa/81kDa/91kDa/93kDa
Observed MW140kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:1000
IHC 1:20 - 1:50
FC 1:20 - 1:50
Storage bufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Flow Cytometry    
Positive samplesU-251MG
Cellular locationCell membrane, Endosome membrane, Single-pass type I membrane protein
Customer validation

WB(Rat)

ABclonal:Western blot - NTRK2 antibody (A0487)}

Western blot - NTRK2 antibody (A0487)

Western blot analysis of extracts of U-251MG cells, using NTRK2 antibody (A0487) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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