Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

NRF2 Rabbit pAb (A1244)

Review (1) Publications (48) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - NRF2 Rabbit pAb (A1244)

Western blot analysis of lysates from NIH/3T3, C6 cells using NRF2 Rabbit pAb (A1244) at 1:1000 dilution. NIH/3T3 cells and C6 cells were treated by MG132(10 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 45 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A1244)

Immunohistochemistry analysis of NRF2 in paraffin-embedded human esophageal cancer using NRF2 Rabbit pAb (A1244) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A1244)

Immunohistochemistry analysis of NRF2 in paraffin-embedded human placenta using NRF2 Rabbit pAb (A1244) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A1244)

Immunohistochemistry analysis of NRF2 in paraffin-embedded mouse kidney using NRF2 Rabbit pAb (A1244) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A1244)

Immunohistochemistry analysis of NRF2 in paraffin-embedded rat kidney using NRF2 Rabbit pAb (A1244) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Review
ABclonal: review for NRF2 Rabbit pAb(A1244)

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Overview

Product name NRF2 Rabbit pAb
Catalog No. A1244
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 243-400 of human NRF2 (NP_006155.2).
Sequence SPHFLNAFEDSFSSILSTEDPNQLTVNSLNSDATVNTDFGDEFYSAFIAEPSISNSMPSPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHSVESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSS
Gene ID 4780
Swiss prot Q16236
Synonyms NRF2; HEBP1; Nrf-2; IMDDHH
Calculated MW 68kDa
Observed MW 100kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples NIH/3T3, C6
Cellular location Cytoplasm, Nucleus, cytosol
Customer validation

WB (Mouse liverRattus norvegicus, Homo sapiens, Mus musculus, Cyprinus carpio, Rattus norvegicus, Rattus)

IF (Mus musculus)

IHC (Mus musculus, Homo sapiens)

WB IF (Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

NRF2 Rabbit pAb images

ABclonal:Western blot - NRF2 Rabbit pAb (A1244)}

Western blot - NRF2 Rabbit pAb (A1244)

Western blot analysis of lysates from NIH/3T3, C6 cells using NRF2 Rabbit pAb (A1244) at 1:1000 dilution. NIH/3T3 cells and C6 cells were treated by MG132(10 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 45 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A1244)}

Immunohistochemistry - NRF2 Rabbit pAb (A1244)

Immunohistochemistry analysis of NRF2 in paraffin-embedded human esophageal cancer using NRF2 Rabbit pAb (A1244) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A1244)}

Immunohistochemistry - NRF2 Rabbit pAb (A1244)

Immunohistochemistry analysis of NRF2 in paraffin-embedded human placenta using NRF2 Rabbit pAb (A1244) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A1244)}

Immunohistochemistry - NRF2 Rabbit pAb (A1244)

Immunohistochemistry analysis of NRF2 in paraffin-embedded mouse kidney using NRF2 Rabbit pAb (A1244) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A1244)}

Immunohistochemistry - NRF2 Rabbit pAb (A1244)

Immunohistochemistry analysis of NRF2 in paraffin-embedded rat kidney using NRF2 Rabbit pAb (A1244) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on NFE2L2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to NFE2L2. (Distance between topics and target gene indicate popularity.) NFE2L2

* Data provided by citexs.com, for reference only.

Publishing research using A1244? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

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