Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

NRF2 Rabbit pAb (A11159)

Publications (12) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - NRF2 Rabbit pAb (A11159)

Western blot analysis of C6, using NRF2 antibody (A11159) at 1:1000 dilution.C6 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry analysis of paraffin-embedded mouse brain using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry analysis of paraffin-embedded rat brain using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - NRF2 Rabbit pAb (A11159)

Immunofluorescence analysis of HeLa cells using NRF2 Rabbit pAb (A11159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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    Overview

    Product nameNRF2 Rabbit pAb
    Catalog No.A11159
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG

    Background

    This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.

    Immunogen information

    ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300-400 of human NRF2 (NP_006155.2).
    SequencePSPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHSVESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSS
    Gene ID4780
    Swiss protQ16236
    SynonymsNRF2; HEBP1; Nrf-2; IMDDHH
    Calculated MW68kDa
    Observed MW97-100kDa

    Applications

    ReactivityHuman, Mouse, Rat
    Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition
    Recommended dilution
    • WB 1:500 - 1:1000
    • IHC-P 1:50 - 1:200
    • IF/ICC 1:50 - 1:200
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
    Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
    Positive samplesC6
    Cellular locationCytoplasm, Nucleus, cytosol
    Customer validation

    WB(Mus musculus, Homo sapiens, Rattus norvegicus, human, Sus scrofa)

    IF(Mus musculus)

    IF(Rattus norvegicus)

    IF/ICC(Sus scrofa)

    IHC(Rattus norvegicus)

    Research Area

    NRF2 Rabbit pAb images

    ABclonal:Western blot - NRF2 Rabbit pAb (A11159)}

    Western blot - NRF2 Rabbit pAb (A11159)

    Western blot analysis of C6, using NRF2 antibody (A11159) at 1:1000 dilution.C6 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.
    ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)}

    Immunohistochemistry - NRF2 Rabbit pAb (A11159)

    Immunohistochemistry analysis of paraffin-embedded human liver cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)}

    Immunohistochemistry - NRF2 Rabbit pAb (A11159)

    Immunohistochemistry analysis of paraffin-embedded human lung cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)}

    Immunohistochemistry - NRF2 Rabbit pAb (A11159)

    Immunohistochemistry analysis of paraffin-embedded mouse brain using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)}

    Immunohistochemistry - NRF2 Rabbit pAb (A11159)

    Immunohistochemistry analysis of paraffin-embedded mouse kidney using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)}

    Immunohistochemistry - NRF2 Rabbit pAb (A11159)

    Immunohistochemistry analysis of paraffin-embedded rat brain using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - NRF2 Rabbit pAb (A11159)}

    Immunofluorescence - NRF2 Rabbit pAb (A11159)

    Immunofluorescence analysis of HeLa cells using NRF2 Rabbit pAb (A11159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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    Related Targets

    Based on existing publications, the following genes are closely related to research on NFE2L2. (Numbers indicate number of published articles where both targets appear.)

    1/

    Related Research Area

    Based on existing publications, the following research topics are related to NFE2L2. (Distance between topics and target gene indicate popularity.) NFE2L2

    * Data provided by citexs.com, for reference only.

    Publishing research using A11159? Please let us know so that we can cite the reference in this datasheet.

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