Publications (10) Datasheet SDS
Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat
Western blot analysis of C6, using NRF2 antibody (A11159) at 1:1120 dilution.C6 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunohistochemistry of paraffin-embedded human liver cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human lung cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse brain using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat brain using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
| Product name | NRF2 Rabbit pAb |
|---|---|
| Catalog No. | A11159 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human NRF2 (NP_006155.2). |
|---|---|
| Sequence | PSPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHSVESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSS |
| Gene ID | 4780 |
| Swiss prot | Q16236 |
| Synonyms | NFE2L2; HEBP1; NRF2 |
| Calculated MW | 65kDa/66kDa/67kDa |
| Observed MW | 97-100kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry |
| Positive samples | C6 |
| Cellular location | Cytoplasm, Nucleus, cytosol |
| Customer validation | WB(Mus musculus, Homo sapiens, Rattus norvegicus, human, Sus scrofa) IF(Mus musculus) IF(Rattus norvegicus) IF/ICC(Sus scrofa) |
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* For research use only. Not for therapeutic or diagnostic purposes.
![[KO Validated] NRF2 Rabbit mAb](https://abclonal.com/Uploads/Catalog/A21176/A21176_1.jpg?t=1667379744)
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