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NRF2 Rabbit pAb (A11159)

Publications (7) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - NRF2 Rabbit pAb (A11159)

Western blot analysis of C6, using NRF2 antibody (A11159) at 1:1120 dilution.C6 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry of paraffin-embedded human breast cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry of paraffin-embedded human liver cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - NRF2 Rabbit pAb (A11159)

Immunofluorescence analysis of HeLa cells using NRF2 Rabbit pAb (A11159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameNRF2 Rabbit pAb
Catalog No.A11159
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300-400 of human NRF2 (NP_006155.2).
SequencePSPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHSVESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSS
Gene ID4780
Swiss protQ16236
SynonymsNFE2L2; HEBP1; NRF2
Calculated MW65kDa/66kDa/67kDa
Observed MW97-100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF 1:50 - 1:200
  • IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesC6
Cellular locationCytoplasm, Nucleus, cytosol
Customer validation

WB(Mus musculus, Homo sapiens)

IF(Mus musculus)

ABclonal:Western blot - NRF2 Rabbit pAb (A11159)}

Western blot - NRF2 Rabbit pAb (A11159)

Western blot analysis of C6, using NRF2 antibody (A11159) at 1:1120 dilution.C6 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)}

Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry of paraffin-embedded human breast cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)}

Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry of paraffin-embedded human liver cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - NRF2 Rabbit pAb (A11159)}

Immunofluorescence - NRF2 Rabbit pAb (A11159)

Immunofluorescence analysis of HeLa cells using NRF2 Rabbit pAb (A11159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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