Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition
Reactivity:Human, Mouse, Rat
Western blot analysis of C6, using NRF2 antibody (A11159) at 1:1000 dilution.C6 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat brain using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
| Product name | NRF2 Rabbit pAb |
|---|---|
| Catalog No. | A11159 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human NRF2 (NP_006155.2). |
|---|---|
| Sequence | PSPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHSVESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSS |
| Gene ID | 4780 |
| Swiss prot | Q16236 |
| Synonyms | NRF2; HEBP1; Nrf-2; IMDDHH |
| Calculated MW | 68kDa |
| Observed MW | 97-100kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | C6 |
| Cellular location | Cytoplasm, Nucleus, cytosol |
| Customer validation | WB(Mus musculus, Homo sapiens, Rattus norvegicus, human, Sus scrofa) IF(Mus musculus) IF(Rattus norvegicus) IF/ICC(Sus scrofa) IHC(Rattus norvegicus) |
Submit your question about A11159 below and we will get back to you within one business day.
Alternatively, call us at 888.754.5670.
Based on existing publications, the following genes are closely related to research on NFE2L2. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to NFE2L2. (Distance between topics and target gene indicate popularity.) NFE2L2
* Data provided by citexs.com, for reference only.
Publishing research using A11159? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.
![[KO Validated] NRF2 Rabbit mAb](https://abclonal-us.oss-us-east-1.aliyuncs.com/abclonal/Catalog/A3577/A3577_1.jpg?t=1692237396)
