Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

NRF2 Rabbit pAb (A11159)

Publications (10) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - NRF2 Rabbit pAb (A11159)

Western blot analysis of C6, using NRF2 antibody (A11159) at 1:1120 dilution.C6 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry of paraffin-embedded human liver cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry of paraffin-embedded human lung cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry of paraffin-embedded mouse brain using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry of paraffin-embedded mouse kidney using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry of paraffin-embedded rat brain using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - NRF2 Rabbit pAb (A11159)

Immunofluorescence analysis of HeLa cells using NRF2 Rabbit pAb (A11159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameNRF2 Rabbit pAb
Catalog No.A11159
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.

Immunogen information

ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300-400 of human NRF2 (NP_006155.2).
SequencePSPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHSVESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSS
Gene ID4780
Swiss protQ16236
SynonymsNFE2L2; HEBP1; NRF2
Calculated MW65kDa/66kDa/67kDa
Observed MW97-100kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesC6
Cellular locationCytoplasm, Nucleus, cytosol
Customer validation

WB(Mus musculus, Homo sapiens, Rattus norvegicus, human, Sus scrofa)

IF(Mus musculus)

IF(Rattus norvegicus)

IF/ICC(Sus scrofa)

Research Area

NRF2 Rabbit pAb images

ABclonal:Western blot - NRF2 Rabbit pAb (A11159)}

Western blot - NRF2 Rabbit pAb (A11159)

Western blot analysis of C6, using NRF2 antibody (A11159) at 1:1120 dilution.C6 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)}

Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry of paraffin-embedded human liver cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)}

Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry of paraffin-embedded human lung cancer using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)}

Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry of paraffin-embedded mouse brain using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)}

Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry of paraffin-embedded mouse kidney using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A11159)}

Immunohistochemistry - NRF2 Rabbit pAb (A11159)

Immunohistochemistry of paraffin-embedded rat brain using NRF2 Rabbit pAb (A11159) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - NRF2 Rabbit pAb (A11159)}

Immunofluorescence - NRF2 Rabbit pAb (A11159)

Immunofluorescence analysis of HeLa cells using NRF2 Rabbit pAb (A11159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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