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NRF2 Rabbit pAb (A0674)

Review (1)Publications (9) Datasheet

ABclonal:Western blot - NRF2 Rabbit pAb (A0674)

Western blot analysis of extracts of NIH/3T3 cells, using NRF2 antibody (A0674) at 1:1000 dilution.NIH/3T3 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.

ABclonal:Western blot - NRF2 Rabbit pAb (A0674)

Western blot analysis of extracts from normal (control) and NRF2 knockout (KO) HeLa cells, using NRF2 antibody (A0674) at 1:1000 dilution.HeLa cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A0674)

Immunohistochemistry of paraffin-embedded rat brain using NRF2 antibody (A0674) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A0674)

Immunohistochemistry of paraffin-embedded human breast cancer using NRF2 antibody (A0674) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A0674)

Immunohistochemistry of paraffin-embedded mouse testis using NRF2 antibody (A0674) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - NRF2 Rabbit pAb (A0674)

Immunofluorescence analysis of NIH/3T3 cells using NRF2 Rabbit pAb (A0674) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NRF2 Rabbit pAb (A0674)

Immunofluorescence analysis of PC-12 cells using NRF2 Rabbit pAb (A0674) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NRF2 Rabbit pAb (A0674)

Immunofluorescence analysis of U2OS cells using NRF2 Rabbit pAb (A0674) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - NRF2 Rabbit pAb (A0674)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug NRF2 antibody (A0674). Western blot was performed from the immunoprecipitate using NRF2 (A0674) at a dilition of 1:1000.

Review
ABclonal: review for NRF2 Rabbit pAb(A0674)

Overview

Product nameNRF2 Rabbit pAb
Catalog No.A0674
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 366-605 of human NRF2 (NP_006155.2).
SequenceYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSSGDMVQPLSPSQGQSTHVHDAQCENTPEKELPVSPGHRKTPFTKDKHSSRLEAHLTRDELRAKALHIPFPVEKIINLPVVDFNEMMSKEQFNEAQLALIRDIRRRGKNKVAAQNCRKRKLENIVELEQDLDHLKDEKEKLLKEKGENDKSLHLLKKQLSTLYLEVFSMLRDEDGKPYSPSEYSLQQTRDGNVFLVPKSKKPDVKKN
Gene ID4780
Swiss protQ16236
SynonymsNFE2L2; HEBP1; NRF2
Calculated MW65kDa/66kDa/67kDa
Observed MW97-100KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, NIH/3T3
Cellular locationCytoplasm, Nucleus, cytosol
Customer validation

(Mouse embryonal fibroblast cell)

WB(Mus musculus, Homo sapiens, Gallus gallus, Rattus norvegicus)

ABclonal:Western blot - NRF2 Rabbit pAb (A0674) }

Western blot - NRF2 Rabbit pAb (A0674)

Western blot analysis of extracts of NIH/3T3 cells, using NRF2 antibody (A0674) at 1:1000 dilution.NIH/3T3 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.
ABclonal:Western blot - NRF2 Rabbit pAb (A0674) }

Western blot - NRF2 Rabbit pAb (A0674)

Western blot analysis of extracts from normal (control) and NRF2 knockout (KO) HeLa cells, using NRF2 antibody (A0674) at 1:1000 dilution.HeLa cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A0674) }

Immunohistochemistry - NRF2 Rabbit pAb (A0674)

Immunohistochemistry of paraffin-embedded rat brain using NRF2 antibody (A0674) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A0674) }

Immunohistochemistry - NRF2 Rabbit pAb (A0674)

Immunohistochemistry of paraffin-embedded human breast cancer using NRF2 antibody (A0674) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - NRF2 Rabbit pAb (A0674) }

Immunohistochemistry - NRF2 Rabbit pAb (A0674)

Immunohistochemistry of paraffin-embedded mouse testis using NRF2 antibody (A0674) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - NRF2 Rabbit pAb (A0674) }

Immunofluorescence - NRF2 Rabbit pAb (A0674)

Immunofluorescence analysis of NIH/3T3 cells using NRF2 Rabbit pAb (A0674) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NRF2 Rabbit pAb (A0674) }

Immunofluorescence - NRF2 Rabbit pAb (A0674)

Immunofluorescence analysis of PC-12 cells using NRF2 Rabbit pAb (A0674) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NRF2 Rabbit pAb (A0674) }

Immunofluorescence - NRF2 Rabbit pAb (A0674)

Immunofluorescence analysis of U2OS cells using NRF2 Rabbit pAb (A0674) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - NRF2 Rabbit pAb (A0674) }

Immunoprecipitation - NRF2 Rabbit pAb (A0674)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug NRF2 antibody (A0674). Western blot was performed from the immunoprecipitate using NRF2 (A0674) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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