Products > Catalog Antibodies > Polyclonal Antibodies

NRF2 Rabbit pAb (A11159)

Publications (2) Datasheet

ABclonal:Western blot - NRF2 Polyclonal Antibody (A11159)
ABclonal:Immunohistochemistry - NRF2 Polyclonal Antibody (A11159)
ABclonal:Immunohistochemistry - NRF2 Polyclonal Antibody (A11159)
ABclonal:Immunofluorescence - NRF2 Polyclonal Antibody (A11159)
ABclonal:Immunofluorescence - NRF2 Polyclonal Antibody (A11159)
ABclonal:Immunofluorescence - NRF2 Polyclonal Antibody (A11159)

Overview

Product nameNRF2 Rabbit pAb
Catalog No.A11159
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 366-605 of human NRF2 (NP_006155.2).
SequenceYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSSGDMVQPLSPSQGQSTHVHDAQCENTPEKELPVSPGHRKTPFTKDKHSSRLEAHLTRDELRAKALHIPFPVEKIINLPVVDFNEMMSKEQFNEAQLALIRDIRRRGKNKVAAQNCRKRKLENIVELEQDLDHLKDEKEKLLKEKGENDKSLHLLKKQLSTLYLEVFSMLRDEDGKPYSPSEYSLQQTRDGNVFLVPKSKKPDVKKN
Gene ID4780
Swiss protQ16236
SynonymsNFE2L2; HEBP1; NRF2
Calculated MW65kDa/66kDa/67kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-549, HepG2, HeLa, Rat liver
Cellular locationCytoplasm, Nucleus, cytosol
Customer validation

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - NRF2 Polyclonal Antibody (A11159) }

Western blot - NRF2 Polyclonal Antibody (A11159)

Western blot analysis of extracts of various cell lines, using NRF2 antibody (A11159) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - NRF2 Polyclonal Antibody (A11159) }

Immunohistochemistry - NRF2 Polyclonal Antibody (A11159)

Immunohistochemistry of paraffin-embedded mouse leydig cells using NRF2 antibody (A11159) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - NRF2 Polyclonal Antibody (A11159) }

Immunohistochemistry - NRF2 Polyclonal Antibody (A11159)

Immunohistochemistry of paraffin-embedded mouse kidney using NRF2 antibody (A11159) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - NRF2 Polyclonal Antibody (A11159) }

Immunofluorescence - NRF2 Polyclonal Antibody (A11159)

Immunofluorescence analysis of HeLa cells using NRF2 antibody (A11159) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NRF2 Polyclonal Antibody (A11159) }

Immunofluorescence - NRF2 Polyclonal Antibody (A11159)

Immunofluorescence analysis of NIH/3T3 cells using NRF2 antibody (A11159) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NRF2 Polyclonal Antibody (A11159) }

Immunofluorescence - NRF2 Polyclonal Antibody (A11159)

Immunofluorescence analysis of PC12 cells using NRF2 antibody (A11159) at dilution of 1:100. Blue: DAPI for nuclear staining.

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