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NR0B2 Rabbit pAb (A1836)

Reviews (3)Publication (1) Datasheet

ABclonal:Western blot - NR0B2 Polyclonal Antibody (A1836)
ABclonal:Western blot - NR0B2 Polyclonal Antibody (A1836)
ABclonal:Immunohistochemistry - NR0B2 Polyclonal Antibody (A1836)
ABclonal:Immunofluorescence - NR0B2 Polyclonal Antibody (A1836)
Review
ABclonal: review for NR0B2 Rabbit pAb(A1836)
Review
ABclonal: review for NR0B2 Rabbit pAb(A1836)
Review
ABclonal: review for NR0B2 Rabbit pAb(A1836)

Overview

Product nameNR0B2 Rabbit pAb
Catalog No.A1836
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-257 of human NR0B2 (NP_068804.1).
SequenceMSTSQPGACPCQGAASRPAILYALLSSSLKAVPRPRSRCLCRQHRPVQLCAPHRTCREALDVLAKTVAFLRNLPSFWQLPPQDQRRLLQGCWGPLFLLGLAQDAVTFEVAEAPVPSILKKILLEEPSSSGGSGQLPDRPQPSLAAVQWLQCCLESFWSLELSPKEYACLKGTILFNPDVPGLQAASHIGHLQQEAHWVLCEVLEPWCPAAQGRLTRVLLTASTLKSIPTSLLGDLFFRPIIGDVDIAGLLGDMLLLR
Gene ID8431
Swiss protQ15466
SynonymsNR0B2; SHP; SHP1
Calculated MW28kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse liver, Rat liver, Rat testis, HepG2, 293T
Cellular locationCytoplasm, Nucleus
Customer validation

(Human chronic myeloid leukemia cells, Murine macrophages cells)

WB(Mus musculus)

ABclonal:Western blot - NR0B2 Polyclonal Antibody (A1836) }

Western blot - NR0B2 Polyclonal Antibody (A1836)

Western blot analysis of extracts of various cell lines, using NR0B2 antibody (A1836) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - NR0B2 Polyclonal Antibody (A1836) }

Western blot - NR0B2 Polyclonal Antibody (A1836)

Western blot analysis of extracts of various cell lines, using NR0B2 antibody (A1836) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - NR0B2 Polyclonal Antibody (A1836) }

Immunohistochemistry - NR0B2 Polyclonal Antibody (A1836)

Immunohistochemistry of paraffin-embedded rat liver using NR0B2 antibody (A1836) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - NR0B2 Polyclonal Antibody (A1836) }

Immunofluorescence - NR0B2 Polyclonal Antibody (A1836)

Immunofluorescence analysis of U2OS cells using NR0B2 antibody (A1836). Blue: DAPI for nuclear staining.

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