Products > Catalog Antibodies > Polyclonal Antibodies

NQO1 Rabbit pAb (A0047)

Review (1)Publications (9) Datasheet

ABclonal:Western blot - NQO1 Rabbit pAb (A0047)

Western blot analysis of extracts of various cell lines, using NQO1 antibody (A0047) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - NQO1 Rabbit pAb (A0047)

Western blot analysis of extracts of Rat liver, using NQO1 antibody (A0047) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - NQO1 Rabbit pAb (A0047)

Immunofluorescence analysis of NIH/3T3 cells using NQO1 Rabbit pAb (A0047) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NQO1 Rabbit pAb (A0047)

Immunofluorescence analysis of PC-12 cells using NQO1 Rabbit pAb (A0047) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - NQO1 Rabbit pAb (A0047)

Immunofluorescence analysis of U2OS cells using NQO1 Rabbit pAb (A0047) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for NQO1 Rabbit pAb(A0047)

Overview

Product nameNQO1 Rabbit pAb
Catalog No.A0047
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human NQO1 (NP_000894.1).
SequenceMVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK
Gene ID1728
Swiss protP15559
SynonymsDHQU; DIA4; DTD; NMOR1; NMORI; QR1; NQO1
Calculated MW26kDa/27kDa/30kDa
Observed MW31KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, HT-29, HepG2, MCF7, Rat liver
Cellular locationCytoplasm
Customer validation

(Huh7.5.1,HepG2)

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

ABclonal:Western blot - NQO1 Rabbit pAb (A0047) }

Western blot - NQO1 Rabbit pAb (A0047)

Western blot analysis of extracts of various cell lines, using NQO1 antibody (A0047) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - NQO1 Rabbit pAb (A0047) }

Western blot - NQO1 Rabbit pAb (A0047)

Western blot analysis of extracts of Rat liver, using NQO1 antibody (A0047) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - NQO1 Rabbit pAb (A0047) }

Immunofluorescence - NQO1 Rabbit pAb (A0047)

Immunofluorescence analysis of NIH/3T3 cells using NQO1 Rabbit pAb (A0047) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NQO1 Rabbit pAb (A0047) }

Immunofluorescence - NQO1 Rabbit pAb (A0047)

Immunofluorescence analysis of PC-12 cells using NQO1 Rabbit pAb (A0047) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - NQO1 Rabbit pAb (A0047) }

Immunofluorescence - NQO1 Rabbit pAb (A0047)

Immunofluorescence analysis of U2OS cells using NQO1 Rabbit pAb (A0047) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A0047

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A0047 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A0047? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μL sample size available Add to Cart
Contact us to order