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NQO1 Rabbit mAb (A19586)

Publication (1) Datasheet

ABclonal:Western blot - NQO1 Rabbit mAb (A19586)
ABclonal:Immunohistochemistry - NQO1 Rabbit mAb (A19586)
ABclonal:Immunoprecipitation - NQO1 Rabbit mAb (A19586)

Overview

Product nameNQO1 Rabbit mAb
Catalog No.A19586
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
ImmunogenA synthesized peptide derived from human NQO1.
SequenceEmail for sequence
Gene ID1728
Swiss protP15559
SynonymsDHQU; DIA4; DTD; NMOR1; NMORI; QR1; NQO1
Calculated MW31kDa
Observed MW31KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesHepG2, HeLa, HT-29, Mouse brain, Mouse liver, Mouse spleen, Rat liver
Cellular locationCytoplasm
Customer validation

WB(Mus musculus)

ABclonal:Western blot - NQO1 Rabbit mAb (A19586) }

Western blot - NQO1 Rabbit mAb (A19586)

Western blot analysis of extracts of various cell lines, using NQO1 antibody (A19586) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - NQO1 Rabbit mAb (A19586) }

Immunohistochemistry - NQO1 Rabbit mAb (A19586)

Immunohistochemistry of paraffin-embedded mouse kidney using NQO1 antibody (A19586) at dilution of 1:100 (40x lens).
ABclonal:Immunoprecipitation - NQO1 Rabbit mAb (A19586) }

Immunoprecipitation - NQO1 Rabbit mAb (A19586)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug NQO1 antibody (A19586). Western blot was performed from the immunoprecipitate using NQO1 antibody (A19586) at a dilition of 1:1000.

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