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NPPA Polyclonal Antibody (A1609)

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ABclonal:Western blot - NPPA Polyclonal Antibody (A1609)
Review
ABclonal: review for NPPA Polyclonal Antibody(A1609)

Overview

Product nameNPPA Polyclonal Antibody
Catalog No.A1609
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 26-151 of human NPPA (NP_006163.1).
SequenceNPMYNAVSNADLMDFKNLLDHLEEKMPLEDEVVPPQVLSEPNEEAGAALSPLPEVPPWTGEVSPAQRDGGALGRGPWDSSDRSALLKSKLRALLTAPRSLRRSSCFGGRMDRIGAQSGLGCNSFRY
Gene ID4878
Swiss protP01160
SynonymsNPPA; ANF; ANP; ATFB6; ATRST2; CDD; CDD-ANF; CDP; PND; natriuretic peptide A
Calculated MW16kDa
Observed MW16kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesmouse brain, mouse heart, mouse lung
Cellular locationSecreted
Customer validation

WB(Rat,Mouse)

IF(Rat,Mouse)

ABclonal:Western blot - NPPA Polyclonal Antibody (A1609) }

Western blot - NPPA Polyclonal Antibody (A1609)

Western blot analysis of extracts of various cell lines, using NPPA antibody (A1609) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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